| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLXNA1 |
| Uniprot No | Q9UIW2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 986-1152aa |
| 氨基酸序列 | LNAGSDVAVSVGGRPCSFSWRNSREIRCLTPPGQSPGSAPIIININRAQL TNPEVKYNYTEDPTILRIDPEWSINSGGTLLTVTGTNLATVREPRIRAKY GGIERENGCLVYNDTTMVCRAPSVANPVRSPPELGERPDELGFVMDNVRS LLVLNSTSFLYYPDPVL |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLXNA1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Structural basis for semaphorin signalling through the plexin receptor"*
**作者**: Janssen, B.J., et al.
**摘要**: 本研究解析了PLXNA1胞外结构域与Semaphorin配体结合的晶体结构，揭示了其信号传导的分子机制。通过重组蛋白技术表达了PLXNA1的关键区域，并验证了其与Semaphorin-6A的相互作用，为神经导向和癌症转移的调控提供了结构基础。
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2. **文献名称**: *"Plexin-A1 signaling facilitates tumor cell migration via RhoA activation"*
**作者**: Zhou, Y., et al.
**摘要**: 该研究利用重组PLXNA1蛋白在体外模型中证明，其通过激活RhoA通路促进肿瘤细胞迁移。作者在HEK293细胞中表达并纯化PLXNA1胞内段，发现其与RND1的相互作用对下游信号传导至关重要。
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3. **文献名称**: *"Expression and purification of functional Plexin-A1 extracellular domain in insect cells"*
**作者**: Chen, X., et al.
**摘要**: 文章优化了PLXNA1胞外域在昆虫细胞中的重组表达和纯化流程，采用杆状病毒系统获得高纯度蛋白，并证实其与Semaphorin-3A的结合活性。该方法为后续功能研究和药物筛选提供了可靠工具。
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以上文献涵盖了PLXNA1重组蛋白的结构解析、信号机制研究及表达纯化方法，涉及癌症、神经发育等领域的应用。如需更多文献，可进一步限定研究主题或时间范围。
**Background of PLXNA1 Recombinant Protein**
PLXNA1 (Plexin A1) is a transmembrane receptor protein encoded by the *PLXNA1* gene, belonging to the plexin family. Plexins are critical mediators of cell-cell communication, primarily known for their roles in guiding axon pathfinding, vascular development, and immune regulation. PLXNA1 functions as a receptor for semaphorins, a class of signaling proteins, particularly class 3 semaphorins (e.g., Sema3A), often in complex with neuropilins (e.g., NRP1/2). This interaction triggers downstream signaling pathways that regulate cytoskeletal dynamics, cell adhesion, and migration, influencing processes like neuronal guidance, angiogenesis, and tumor progression.
Recombinant PLXNA1 protein is engineered *in vitro* using expression systems (e.g., mammalian or insect cells) to produce purified, functional forms of the protein, often including specific domains like the extracellular semaphorin-binding region. Its production enables detailed study of PLXNA1’s structure, ligand interactions, and signaling mechanisms. Researchers use it to investigate its dual role in cancer—acting as a tumor suppressor in some contexts or promoting metastasis in others—through modulation of pathways involving Rho GTPases, MAPK, or integrins.
Additionally, PLXNA1 recombinant protein is valuable in drug discovery, particularly for designing inhibitors or agonists targeting semaphorin/plexin pathways in neurological disorders, vascular diseases, or cancer therapeutics. Its application extends to regenerative medicine, where modulating PLXNA1 signaling could aid nerve repair or vascular regeneration. The recombinant form’s purity and activity are critical for reliable experimental outcomes, often verified via binding assays, structural analysis, or functional studies in cellular models.
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