| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACADM |
| Uniprot No | P11310 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-421aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMKANRQREPGLGFSFEFTEQQKEFQATARK FAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFD ACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPL MCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLL ARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFED VKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYAL ERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYA SIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTS QIQRLIVAREHIDKYKN |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACADM重组蛋白的3篇代表性文献摘要概述：
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1. **文献名称**：*Heterologous Expression and Purification of Human Mitochondrial Medium-Chain Acyl-CoA Dehydrogenase (ACADM)*
**作者**：Zhang et al. (2018)
**摘要**：研究利用大肠杆菌系统成功表达并纯化具有活性的人源ACADM重组蛋白，优化了表达条件以提高溶解度，并通过质谱和圆二色光谱验证其正确折叠，为酶功能研究提供工具。
2. **文献名称**：*Crystal Structure of Recombinant Human ACADM Reveals Insights into Substrate Binding*
**作者**：Thompson & Lee (2020)
**摘要**：通过X射线晶体学解析了重组ACADM蛋白的3D结构(分辨率2.1Å)，揭示了底物结合口袋的关键氨基酸残基，为理解突变导致的代谢疾病(如MCAD缺乏症)提供分子机制。
3. **文献名称**：*Functional Characterization of ACADM Variants Using Recombinant Protein Assays*
**作者**：Garcia et al. (2021)
**摘要**：构建了多个ACADM致病突变体的重组蛋白，通过体外酶活实验发现特定突变导致催化活性丧失或降低，结合热稳定性分析，为临床基因型-表型关联提供生化证据。
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**备注**：以上文献为示例性内容，实际引用时需核实具体发表信息及数据库收录情况(如PubMed ID)。建议通过关键词“ACADM recombinant expression”或“MCAD purification”在专业平台检索最新研究。
**Background of ACADM Recombinant Protein**
ACADM (acyl-CoA dehydrogenase medium chain) is a mitochondrial enzyme critical in fatty acid β-oxidation, catalyzing the dehydrogenation of medium-chain acyl-CoA derivatives to generate energy. Mutations in the *ACADM* gene cause medium-chain acyl-CoA dehydrogenase deficiency (MCADD), an autosomal recessive disorder characterized by impaired fatty acid metabolism, leading to hypoglycemia, liver dysfunction, and life-threatening metabolic crises if untreated.
Recombinant ACADM protein is produced via genetic engineering, typically using expression systems like *E. coli*, yeast, or mammalian cells. Its production enables detailed study of ACADM’s structure, function, and interaction with substrates or inhibitors. Researchers employ it to model MCADD pathogenesis, screen potential therapeutics, and develop enzyme replacement strategies.
The recombinant protein retains enzymatic activity when purified, allowing kinetic assays to assess mutations’ effects on catalytic efficiency. It also aids in generating antibodies for diagnostic kits and validating gene therapy outcomes. Furthermore, structural studies using recombinant ACADM provide insights into mutation-induced conformational changes, guiding drug design.
Advances in recombinant technology, such as codon optimization and fusion tags, enhance protein yield and stability. ACADM recombinant tools are pivotal in bridging biochemical research with clinical applications, offering hope for improved diagnostics, neonatal screening, and targeted therapies for MCADD.
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