| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF3A |
| Uniprot No | P56537 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-245aa |
| 氨基酸序列 | MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD TVQIRRVEER LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN DWCAFCGLDT TSTELSVVES VFKLNEAQPS TIATSMRDSL IDSLT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF3A重组蛋白的3篇代表性文献示例(内容基于假设性研究整理)：
1. **标题**：*Expression and Functional Analysis of Recombinant Human EIF3A in Translation Initiation*
**作者**：Zhang Y, et al.
**摘要**：报道了重组人EIF3A蛋白在大肠杆菌中的表达与纯化方法，并证实其通过与核糖体亚基的相互作用调控翻译起始的分子机制。
2. **标题**：*Structural Insights into EIF3A Role in Cancer via Recombinant Protein Crystallography*
**作者**：Lee S, et al.
**摘要**：通过X射线晶体学解析了重组EIF3A蛋白的三维结构，揭示了其特定结构域在肿瘤细胞异常翻译调控中的关键作用。
3. **标题**：*EIF3A Recombinant Protein Enhances Chemoresistance in Ovarian Cancer Cells*
**作者**：Wang H, et al.
**摘要**：利用重组EIF3A蛋白体外实验，证明其过表达通过激活mTOR通路促进卵巢癌细胞对化疗药物的抵抗性。
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**注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science以关键词“EIF3A recombinant protein”检索最新研究，优先选择近五年内发表的论文以获取前沿信息。
**Background of EIF3A Recombinant Protein**
Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A) is a critical component of the eukaryotic translation initiation factor 3 (eIF3) complex, which plays a central role in the initiation phase of protein synthesis. As the largest subunit of the eIF3 complex, EIF3A is essential for assembling the 43S pre-initiation complex, facilitating ribosomal recruitment to mRNA, and scanning for the start codon. It interacts with other initiation factors, ribosomes, and mRNA to regulate translation efficiency and specificity, particularly under stress conditions or during cell cycle progression. Dysregulation of EIF3A has been linked to various cancers, neurodegenerative diseases, and developmental disorders, highlighting its importance in cellular homeostasis.
Recombinant EIF3A protein is engineered using expression systems like *E. coli* or mammalian cell cultures, enabling large-scale production for functional and structural studies. Its recombinant form retains the ability to integrate into the eIF3 complex, making it invaluable for dissecting translation mechanisms, studying protein-protein or protein-RNA interactions, and screening for therapeutic agents targeting translation dysregulation. Structural analyses using recombinant EIF3A, such as cryo-EM or X-ray crystallography, have provided insights into its role in mRNA recruitment and ribosome assembly.
Additionally, EIF3A’s overexpression in certain cancers has spurred interest in its potential as a diagnostic marker or therapeutic target. Recombinant EIF3A aids in developing inhibitors to modulate aberrant translation in malignancies. Ongoing research continues to explore its regulatory roles in gene-specific translation and stress responses, positioning it as a key molecule in both basic and applied biomedical sciences.
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