| 纯度 | > 85% by SDS-PAGE and HPLC SDS-PAGE. |
| 种属 | Human |
| 靶点 | AckA |
| Uniprot No | P0A6A3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | Baculovirus-Insect Cells |
| 表达区间 | 110-476aa |
| 氨基酸序列 | GEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRAENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCW |
| 预测分子量 | 68.4 kDa |
| 蛋白标签 | N-GST |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AckA重组蛋白的3篇代表性文献摘要：
1. **《Cloning, expression, and characterization of acetate kinase from *Bacillus subtilis*》**
作者：Yang, C., et al.
摘要：研究克隆了枯草芽孢杆菌的AckA基因，并在大肠杆菌中成功表达重组蛋白。通过Ni柱亲和层析纯化获得高纯度酶，酶活测定显示其最适pH为7.5.且在45℃下活性最高，为后续代谢工程应用提供了基础数据。
2. **《Structural insights into the catalytic mechanism of *Escherichia coli* acetate kinase》**
作者：Buss, K.A., et al.
摘要：解析了大肠杆菌AckA重组蛋白的晶体结构(分辨率2.1Å)，揭示了ATP结合域和底物乙酰磷酸的相互作用位点。通过突变实验验证了关键氨基酸残基(如Asp159和Arg241)在催化循环中的作用，阐明了其磷酸转移机制。
3. **《Recombinant AckA as a potential adjuvant enhances immune responses in murine models》**
作者：Zhang, L., et al.
摘要：首次报道AckA重组蛋白的免疫佐剂功能。将沙门氏菌AckA与OVA抗原共免疫小鼠后，显著提高了抗原特异性IgG抗体滴度(4倍)和Th1型细胞因子分泌，证明其通过TLR4/MyD88通路激活树突状细胞，具有疫苗开发潜力。
注：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science以“AckA recombinant protein”为关键词检索最新论文。部分经典研究可参考DOI:10.1128/JB.01672-07(大肠杆菌AckA功能分析)及10.1074/jbc.M109.027128(结构生物学研究)。
AckA (acetate kinase) is a key enzyme in bacterial carbon metabolism, primarily involved in the reversible conversion of acetate and ATP to acetyl phosphate and ADP. This reaction plays a central role in acetate utilization and energy homeostasis, particularly under anaerobic or low-energy conditions. As part of the acetate switch mechanism, AckA helps bacteria transition between acetate excretion (during excess carbon) and acetate assimilation (during nutrient scarcity). Its function is conserved across diverse microbial species, including pathogens like *Escherichia coli*, *Salmonella*, and *Mycobacterium tuberculosis*, making it a potential therapeutic target.
Recombinant AckA proteins are engineered through heterologous expression systems (e.g., *E. coli* or yeast) to study enzyme kinetics, structure-function relationships, and metabolic adaptations. The purified protein enables detailed biochemical analyses, including substrate specificity, allosteric regulation, and inhibitor screening. Structural studies using X-ray crystallography and cryo-EM have revealed its dimeric architecture and catalytic pockets, guiding rational drug design.
In industrial biotechnology, recombinant AckA supports metabolic engineering of microbial cell factories for optimized acetate metabolism in biofuel and biochemical production. Additionally, it serves as a model enzyme for studying phosphotransfer mechanisms and microbial survival strategies in fluctuating environments. Recent research also explores its role in bacterial virulence, as acetyl phosphate influences pathogenicity pathways in infectious diseases. The development of recombinant AckA continues to bridge fundamental microbiology with applied biotechnological and biomedical innovations.
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