| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACOT7 |
| Uniprot No | O00154 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MARPGLIHSA PGLPDTCALL QPPAASAAAA PSMSGPDVET PSAIQICRIM RPDDANVAGN VHGGTILKMI EEAGAIISTR HCNSQNGERC VAALARVERT DFLSPMCIGE VAHVSAEITY TSKHSVEVQV NVMSENILTG AKKLTNKATL WYVPLSLKNV DKVLEVPPVV YSRQEQEEEG RKRYEAQKLE RMETKWRNGD IVQPVLNPEP NTVSYSQSSL IHLVGPSDCT LHGFVHGGVT MKLMDEVAGI VAARHCKTNI VTASVDAINF HDKIRKGCVI TISGRMTFTS NKSMEIEVLV DADPVVDSSQ KRYRAASAFF TYVSLSQEGR SLPVPQLVPE TEDEKKRFEE GKGRYLQMKA KRQGHAEPQP |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACOT7重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"Recombinant expression and functional characterization of human acyl-CoA thioesterase 7 (ACOT7) in E. coli"*
**作者**: Smith J, et al.
**摘要**: 本研究报道了人源ACOT7在大肠杆菌中的重组表达与纯化，通过酶动力学分析揭示了其对长链酰基辅酶A的特异性水解活性，为脂代谢研究提供工具蛋白。
2. **文献名称**: *"Structural insights into ACOT7's catalytic mechanism by X-ray crystallography"*
**作者**: Tanaka K, et al.
**摘要**: 通过重组ACOT7蛋白的结晶和X射线衍射分析，解析了其三维结构及活性位点关键氨基酸残基，阐明了其硫酯酶活性的分子机制。
3. **文献名称**: *"ACOT7 regulates neuroinflammation via NADPH oxidase in microglia: Evidence from recombinant protein overexpression models"*
**作者**: Lee S, et al.
**摘要**: 利用哺乳动物细胞重组表达ACOT7蛋白，发现其通过调控NADPH氧化酶活性影响小胶质细胞炎症反应，提示其在神经退行性疾病中的潜在作用。
注：上述文献为示例性质，实际引用时需核实具体论文信息。建议通过PubMed或Web of Science以“ACOT7 recombinant”为关键词检索最新研究。
**Background of ACOT7 Recombinant Protein**
Acyl-CoA thioesterase 7 (ACOT7), also known as cytosolic thioesterase 2 (CTE-2), is a key enzyme involved in lipid metabolism. It belongs to the acyl-CoA thioesterase (ACOT) family, which catalyzes the hydrolysis of acyl-CoA molecules into free fatty acids and coenzyme A (CoASH). This reaction regulates cellular levels of acyl-CoA esters, critical intermediates in lipid biosynthesis, energy production, and signaling pathways. ACOT7 specifically targets medium- to long-chain acyl-CoAs (C12-C20), influencing lipid homeostasis and mitigating lipotoxicity by controlling acyl-CoA accumulation.
ACOT7 is predominantly expressed in metabolically active tissues, including the brain, liver, and muscle. Its cytosolic localization distinguishes it from mitochondrial or peroxisomal ACOT isoforms, suggesting unique roles in cytoplasmic lipid processing. Studies link ACOT7 to neurodegenerative diseases, such as Alzheimer’s, where dysregulated lipid metabolism may contribute to pathology. It also interacts with peroxisome proliferator-activated receptors (PPARs), connecting lipid metabolism to transcriptional regulation.
Recombinant ACOT7 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Purified ACOT7 enables *in vitro* assays to analyze substrate specificity, enzymatic kinetics, and inhibitor screening. Its recombinant form is vital for investigating metabolic disorders, cancer (where lipid reprogramming supports tumor growth), and drug development targeting ACOT7-related pathways. Structural studies using recombinant protein further elucidate its catalytic mechanism and substrate-binding sites.
In summary, ACOT7 recombinant protein serves as a crucial tool to dissect lipid metabolism dynamics, disease mechanisms, and therapeutic opportunities tied to acyl-CoA regulation.
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