| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACOT8 |
| Uniprot No | O14734 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSSPQAP EDGQGCGDRG DPPGDLRSVL VTTVLNLEPL DEDLFRGRHY WVPAKRLFGG QIVGQALVAA AKSVSEDVHV HSLHCYFVRA GDPKLPVLYQ VERTRTGSSF SVRSVKAVQH GKPIFICQAS FQQAQPSPMQ HQFSMPTVPP PEELLDCETL IDQYLRDPNL QKRYPLALNR IAAQEVPIEI KPVNPSPLSQ LQRMEPKQMF WVRARGYIGE GDMKMHCCVA AYISDYAFLG TALLPHQWQH KVHFMVSLDH SMWFHAPFRA DHWMLYECES PWAGGSRGLV HGRLWRQDGV LAVTCAQEGV IRVKPQVSES KL |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACOT8重组蛋白的3篇参考文献示例：
1. **文献名称**：*"Characterization of human acyl-CoA thioesterase 8 (ACOT8): Substrate specificity and enzymatic activity in vitro"*
**作者**：Hunt, M.C., et al.
**摘要**：该研究通过重组表达人源ACOT8蛋白，分析了其对不同酰基辅酶A底物的水解活性，发现ACOT8对长链和支链酰基辅酶A具有特异性，并揭示了其在中链脂肪酸代谢中的潜在作用。
2. **文献名称**：*"Structural insights into the catalytic mechanism of ACOT8 through X-ray crystallography"*
**作者**：Smith, J., et al.
**摘要**：研究利用重组ACOT8蛋白解析了其晶体结构，揭示了活性位点关键氨基酸残基的构象，阐明了其催化酰基辅酶A水解的分子机制，并提出底物结合口袋的变构调节模型。
3. **文献名称**：*"Functional role of ACOT8 in peroxisomal lipid metabolism: Evidence from recombinant protein studies"*
**作者**：Zhang, L., et al.
**摘要**：通过体外重组ACOT8实验，证实其在过氧化物酶体中参与调控酰基辅酶A的稳态，并发现与过氧化物酶体膜蛋白PEX19的相互作用，提示ACOT8在脂质代谢疾病中的潜在病理关联。
(注：以上文献为示例，实际引用需以真实发表的论文为准。)
ACOT8 (Acyl-CoA Thioesterase 8) is a member of the acyl-CoA thioesterase family, a group of enzymes that catalyze the hydrolysis of acyl-CoA thioester bonds to produce free fatty acids and Coenzyme A (CoASH). This enzyme plays a critical role in regulating intracellular lipid metabolism by controlling the availability of acyl-CoA substrates, which are essential intermediates in fatty acid oxidation, synthesis, and membrane remodeling. ACOT8 is localized primarily in peroxisomes and mitochondria, where it exhibits broad substrate specificity for medium- to long-chain acyl-CoA esters (C12-C20). Its activity influences cellular energy homeostasis, lipid signaling, and the balance between fatty acid storage and utilization.
The recombinant ACOT8 protein is produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to enable detailed biochemical and functional studies. Recombinant expression allows researchers to purify the protein in large quantities for structural analysis (e.g., X-ray crystallography), enzymatic assays, and inhibitor screening. Studies have shown that ACOT8 may participate in diverse physiological processes, including inflammation, oxidative stress response, and apoptosis, by modulating lipid-derived signaling molecules like palmitoylethanolamide (PEA) or arachidonic acid derivatives.
Dysregulation of ACOT8 has been implicated in metabolic disorders, neurodegenerative diseases, and cancer. For instance, altered ACOT8 expression is observed in obesity and type 2 diabetes, suggesting its role in lipid-induced insulin resistance. In cancer, ACOT8 may promote tumor survival by redirecting fatty acids toward energy production or membrane synthesis. Recombinant ACOT8 serves as a valuable tool for elucidating these mechanisms and identifying therapeutic targets. Recent research also explores its potential as a biomarker or drug candidate, particularly in diseases linked to peroxisomal dysfunction or mitochondrial lipid overload.
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