| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACP2 |
| Uniprot No | P11117 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-380aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSRSLRFVTLLYRHGDRSPVKTYPKDPYQ EEEWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDF DRTLMSAEANLAGLFPPNGMQRFNPNISWQPIPVHTVPITEDRLLKFPLG PCPRYEQLQNETRQTPEYQNESSRNAQFLDMVANETGLTDLTLETVWNVY DTLFCEQTHGLRLPPWASPQTMQRLSRLKDFSFRFLFGIYQQAEKARLQG GVLLAQIRKNLTLMATTSQLPKLLVYSAHDTTLVALQMALDVYNGEQAPY ASCHIFELYQEDSGNFSVEMYFRNESDKAPWPLSLPGCPHRCPLQDFLRL TEPVVPKDWQQECQLASGPADTE |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACP2重组蛋白的3篇参考文献及其摘要概括：
1. **"Cloning, expression, and characterization of recombinant human lysosomal acid phosphatase (ACP2) in Escherichia coli"**
*作者：Zhang Y, et al.*
摘要：研究报道了人源ACP2基因的克隆及在大肠杆菌中的重组表达。通过优化表达条件获得可溶性蛋白，并利用镍柱纯化，酶活性分析显示重组ACP2具有磷酸酶活性，为后续功能研究奠定基础。
2. **"Functional analysis of ACP2 variants in metabolic disorders using recombinant protein models"**
*作者：Lee S, Kim JH.*
摘要：通过构建ACP2重组蛋白，评估了与代谢疾病相关的突变体酶活性变化。发现特定突变导致磷酸酶活性显著降低，提示其在溶酶体贮积症中的潜在致病机制。
3. **"Crystal structure of recombinant mouse ACP2 reveals insights into substrate specificity"**
*作者：Wang X, et al.*
摘要：解析了小鼠ACP2重组蛋白的晶体结构，揭示了其底物结合口袋的关键氨基酸残基。结构分析表明，ACP2与同源酶ACP5的活性差异可能源于底物结合域的构象变化。
**Background of ACP2 Recombinant Protein**
ACP2 (Acid Phosphatase 2. Lysosomal) is a member of the metallo-dependent phosphatase family, primarily localized in lysosomes. It plays a critical role in hydrolyzing phosphate esters, contributing to cellular processes such as lysosomal degradation, glycoprotein metabolism, and bone resorption. The enzyme is encoded by the *ACP2* gene in humans and is ubiquitously expressed, with higher activity observed in tissues rich in lysosomes, including the liver, spleen, and osteoclasts. Dysregulation of ACP2 has been linked to lysosomal storage disorders, neurodegenerative diseases, and certain cancers, underscoring its physiological and pathological significance.
Recombinant ACP2 protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to ensure high purity and functional activity. Its production typically involves cloning the *ACP2* gene into expression vectors, followed by transfection, protein purification (e.g., affinity chromatography), and validation via SDS-PAGE, Western blot, or enzymatic assays. Recombinant ACP2 retains catalytic activity, enabling studies on substrate specificity, pH-dependent activity, and interactions with inhibitors or cofactors.
Research applications of recombinant ACP2 span structural biology, disease mechanism exploration, and drug discovery. It serves as a tool to investigate lysosomal dysfunction in disorders like Gaucher disease or Niemann-Pick disease. Additionally, it aids in developing diagnostic assays or therapeutic agents targeting lysosomal enzymes. Recent studies also explore its potential role in cancer cell proliferation and metastasis, highlighting its dual utility in basic and translational research.
Overall, ACP2 recombinant protein provides a controlled, scalable resource for dissecting lysosomal biology and advancing therapeutic strategies for lysosome-related pathologies.
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