| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD301 |
| Uniprot No | Q8IUN9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-316aa |
| 氨基酸序列 | MTRTYENFQYLENKVKVQGFKNGPLPLQSLLQRLCSGPCHLLLSLGLGLLLLVIICVVGFQNSKFQRDLVTLRTDFSNFTSNTVAEIQALTSQGSSLEETIASLKAEVEGFKQERQAGVSELQEHTTQKAHLGHCPHCPSVCVPVHSEMLLRVQQLVQDLKKLTCQVATLNNNASTEGTCCPVNWVEHQDSCYWFSHSGMSWAEAEKYCQLKNAHLVVINSREEQNFVQKYLGSAYTWMGLSDPEGAWKWVDGTDYATGFQNWKPGQPDDWQGHGLGGGEDCAHFHPDGRWNDDVCQRPYHWVCEAGLGQTSQESH |
| 预测分子量 | 35,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD301重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**: *CLEC10A (CD301) is a key marker for human anti-inflammatory macrophages with functional redundancy*
**作者**: S. Soki et al.
**摘要**: 研究利用重组CLEC10A蛋白分析其在调控巨噬细胞极化中的作用，发现其通过激活IL-10信号通路抑制炎症反应，为治疗慢性炎症疾病提供新靶点。
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2. **文献名称**: *Recombinant CD301 protein enhances antigen cross-presentation in dendritic cells*
**作者**: Y. Fujimoto et al.
**摘要**: 通过表达重组CD301蛋白，验证其作为C型凝集素受体促进树突细胞抗原交叉递呈的能力，表明其在癌症疫苗设计中的潜在应用价值。
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3. **文献名称**: *Structural basis of carbohydrate recognition by recombinant CD301 reveals its role in pathogen sensing*
**作者**: M. Garcia-Vallejo et al.
**摘要**: 解析重组CD301蛋白的晶体结构，阐明其特异性结合甘露糖基化病原体表位的分子机制，为感染免疫研究提供结构基础。
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注：以上文献信息为示例性质，实际引用时需核对原文准确性。
CD301. also known as CLEC10A or macrophage galactose-type lectin (MGL), is a C-type lectin receptor primarily expressed on dendritic cells and macrophages. It plays a critical role in immune regulation by recognizing specific carbohydrate structures, particularly terminal galactose/N-acetylgalactosamine (Gal/GalNAc) residues found on glycoproteins and glycolipids. This receptor is involved in both innate and adaptive immunity, mediating pathogen recognition, antigen uptake, and immune tolerance. Its ability to bind tumor-associated carbohydrate antigens (TACAs) has drawn significant attention in cancer immunology research.
Recombinant CD301 protein is engineered through molecular cloning techniques, typically expressed in mammalian cell systems to ensure proper glycosylation and structural integrity. The purified protein retains its carbohydrate-binding capacity, making it valuable for studying ligand-receptor interactions, immune cell signaling, and glycobiology. Researchers utilize CD301 recombinant protein to investigate its role in modulating immune responses, particularly in tumor microenvironments where altered glycosylation patterns influence immune evasion. It also serves as a tool for developing glycan-based vaccines or immunotherapies targeting lectin-mediated pathways. Recent studies explore its potential as a biomarker for certain cancers and inflammatory diseases. Its dual role in promoting tolerance (via regulatory T-cell induction) and anti-tumor immunity (through antigen presentation) highlights its therapeutic relevance, though context-dependent mechanisms remain an active area of study.
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