| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRPM8 |
| Uniprot No | Q7Z2W7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 690-1104aa |
| 氨基酸序列 | TKNWKIILCLFIIPLVGCGFVSFRKKPVDKHKKLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHPPELVLYSLVFVLFCDEVRQWYVNGVNYFTDLWNVMDTLGLFYFIAGIVFRLHSSNKSSLYSGRVIFCLDYIIFTLRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEQRWRWIFRSVIYEPYLAMFGQVPSDVDGTTYDFAHCTFTGNESKPLCVELDEHNLPRFPEWITIPLVCIYMLSTNILLVNLLVAMFGYTVGTVQENNDQVWKFQRYFLVQEYCSRLNIPFPFIVFAYFYMVVKKCFKCCCKEKNMESSVCCFKNEDNETLAWEGVMKENYLVKINTKANDTSEEMRHRFRQLDTKLNDLKGLLKEIANKIK |
| 预测分子量 | 50.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRPM8重组蛋白的3篇代表性文献摘要：
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1. **文献名称**：*TRPM8 is a neuronal cold sensor whose activation is potentiated by menthol*
**作者**：McKemy DD, Neuhausser WM, Julius D
**摘要**：该研究首次克隆并鉴定了TRPM8作为冷敏感离子通道的功能。通过在HEK293细胞中重组表达TRPM8蛋白，发现其可被低温(<28℃)和薄荷醇激活，揭示了其在冷感知神经信号传递中的关键作用。
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2. **文献名称**：*Structure of the cold- and menthol-sensing ion channel TRPM8*
**作者**：Yin Y, Wu M, Zubcevic L et al.
**摘要**：本研究通过冷冻电镜技术解析了人源TRPM8重组蛋白的3.4Å分辨率结构，揭示了其结合薄荷醇和磷脂分子PIP2的位点，阐明了温度与化学配体协同调控通道活性的分子机制。
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3. **文献名称**：*Pharmacological characterization of recombinant TRPM8 ion channels using calcium microplate assays*
**作者**：Bödding M, Wissenbach U, Flockerzi V
**摘要**：开发了一种基于重组TRPM8蛋白的高通量钙离子检测方法，系统评估了薄荷醇、icilin等激动剂及多种拮抗剂的效价，为TRPM8靶向药物筛选提供了可靠平台。
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这些研究覆盖了TRPM8的功能发现、结构解析及重组蛋白应用，均通过重组表达技术实现了分子机制探索。如需具体发表信息，建议通过PubMed或Web of Science检索标题或作者进一步确认。
TRPM8 (Transient Receptor Potential Melastatin 8) is a calcium-permeable non-selective cation channel belonging to the TRP superfamily, first identified as a prostate-specific protein. It is best known as a cold sensor activated by temperatures below 26°C and as the molecular target of cooling agents like menthol and icilin. TRPM8 plays critical roles in thermoregulation, cold-induced pain signaling, and inflammatory responses. Structurally, it functions as a tetramer, with each subunit containing six transmembrane domains and cytosolic N- and C-termini involved in ligand binding and channel modulation.
Recombinant TRPM8 proteins are engineered to study its biophysical properties, ligand interactions, and regulatory mechanisms. These proteins are typically expressed in heterologous systems (e.g., HEK293 cells or insect cells) using plasmid vectors, often tagged with fluorescent markers or affinity tags (e.g., GFP, His-tag) for purification and visualization. Purified TRPM8 is utilized in electrophysiology (patch-clamp), calcium imaging, and cryo-EM studies to resolve its structure-function relationships. Recent cryo-EM structures (resolved at ~3-4 Å) have revealed conformational changes during cooling compound activation and phosphatidylinositol 4.5-bisphosphate (PIP2)-dependent gating.
Pharmaceutically, TRPM8 is a therapeutic target for chronic pain (e.g., neuropathic pain), migraine, and overactive bladder. Recombinant proteins enable high-throughput screening of agonists/antagonists, with clinical candidates like PF-05105679 undergoing trials. Challenges remain in maintaining protein stability during purification due to its temperature-sensitive activity and complex membrane topology. Ongoing research focuses on optimizing expression systems and developing nanodisc-embedded TRPM8 for functional studies. Its role in cancer progression (e.g., prostate, pancreatic) further underscores its biomedical relevance, driving demand for reliable recombinant tools to decode pathophysiology and therapeutic potential.
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