| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2F1 |
| Uniprot No | P35269 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-517aa |
| 氨基酸序列 | AALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL RVNGKSGRKF KGIKKGGVTE NTSYYIFTQC PDGAFEAFPV HNWYNFTPLA RHRTLTAEEA EEEWERRNKV LNHFSIMQQR RLKDQDQDED EEEKEKRGRR KASELRIHDL EDDLEMSSDA SDASGEEGGR VPKAKKKAPL AKGGRKKKKK KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SQEEPESKAK APQQEEGPKG VDEQSDSSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSEESDSSEE SDIDSEASSA LFMAKKKTPP KRERKPSGGS SRGNSRPGTP SAEGGSTSST LRAAASKLEQ GKRVSEMPAA KRLRLDTGPQ SLSGKSTPQP PSGKTTPNSG DVQVTEDAVR RYLTRKPMTT KDLLKKFQTK KTGLSSEQTV NVLAQILKRL NPERKMINDK MHFSLKE |
| 预测分子量 | 58,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GTF2F1重组蛋白的3篇代表性文献的简要总结(基于公开研究领域知识，具体文献需以实际检索为准)：
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1. **文献名称**：*Purification and characterization of human GTF2F1 produced in a recombinant expression system*
**作者**：Conaway RC, Conaway JW
**摘要**：该研究报道了通过大肠杆菌表达系统重组生产人源GTF2F1蛋白，并优化了纯化流程。实验证明重组蛋白在体外转录系统中能有效恢复RNA聚合酶II的转录活性，验证了其与TFIIH等因子的相互作用。
2. **文献名称**：*Structural insights into the role of GTF2F1 in transcription initiation complex assembly*
**作者**：Thomas MC, Chiang CM
**摘要**：通过冷冻电镜和X射线晶体学技术，解析了GTF2F1重组蛋白在RNA聚合酶II转录起始复合体中的三维结构。研究发现，重组GTF2F1通过特定结构域介导DNA模板链的定位，并稳定转录起始前复合物。
3. **文献名称**：*Functional analysis of GTF2F1 mutations in transcriptional regulation*
**作者**：Flores O, Reinberg D
**摘要**：利用重组GTF2F1蛋白进行点突变实验，发现其N端结构域对招募TFIIB及促进启动子逃逸至关重要。研究揭示了GTF2F1在转录起始早期阶段的关键调控机制。
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**注**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等学术平台检索确认。如需具体文献DOI或发表年份，建议使用关键词“GTF2F1 recombinant protein”或“GTF2F1 purification”进一步筛选。
**Background of GTF2F1 Recombinant Protein**
GTF2F1 (General Transcription Factor IIF subunit 1), also known as TFIIF-alpha, is a critical component of the RNA polymerase II transcription machinery. It forms a heterodimer with GTF2F2 (TFIIF-beta) to constitute the general transcription factor IIF (TFIIF), which plays a pivotal role in transcription initiation and elongation. TFIIF facilitates promoter escape by stabilizing the interaction between RNA polymerase II and the DNA template, while also regulating the transition from initiation to elongation phases.
The recombinant GTF2F1 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems*,* followed by purification to ensure high specificity and activity. Its recombinant form allows researchers to study TFIIF's structural and functional roles in vitro, including its interactions with other transcription factors (e.g., TFIIB, TFIIH) or nucleic acids.
Studies involving GTF2F1 recombinant protein have provided insights into transcriptional dysregulation in diseases. For example, mutations or altered expression of GTF2F1 are linked to cancers, such as squamous cell carcinoma and esophageal cancer, where aberrant transcription contributes to oncogenesis. Additionally, recombinant GTF2F1 is utilized in drug discovery to screen compounds targeting transcription machinery, particularly for cancers dependent on hyperactive transcriptional programs.
Beyond cancer, GTF2F1 is implicated in viral infections (e.g., HIV), as viral replication often hijacks host transcriptional machinery. The recombinant protein serves as a tool to dissect these mechanisms and develop antiviral strategies.
Overall, GTF2F1 recombinant protein is indispensable for elucidating fundamental transcriptional processes and developing therapies targeting transcription-related pathologies. Its applications span structural biology, biochemical assays, and disease modeling, underscoring its versatility in biomedical research.
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