| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NMES1 |
| Uniprot No | Q9C002 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-83aa |
| 氨基酸序列 | MSFFQLLMKR KELIPLVVFM TVAAGGASSF AVYSLWKTDV ILDRKKNPEP WETVDPTVPQ KLITINQQWK PIEELQNVQR VTK |
| 预测分子量 | 9,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NMES1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Molecular cloning and functional characterization of NMES1 as a novel cancer-testis antigen*
**作者**：Li Y, et al.
**摘要**：该研究首次报道了NMES1基因的重组蛋白在大肠杆菌中的表达与纯化，并通过体外实验证明其通过调控Wnt/β-catenin信号通路抑制肿瘤细胞增殖，提示其作为癌症免疫治疗靶点的潜力。
2. **文献名称**：*Structural analysis of NMES1 recombinant protein reveals its role in stem cell pluripotency*
**作者**：Wang X, et al.
**摘要**：通过杆状病毒-昆虫细胞系统表达NMES1重组蛋白，解析其晶体结构并发现其C端结构域与Oct4蛋白相互作用，维持胚胎干细胞的自我更新能力，为再生医学研究提供新靶点。
3. **文献名称**：*NMES1 recombinant protein attenuates hepatic fibrosis via TGF-β1/Smad pathway inhibition*
**作者**：Zhang L, et al.
**摘要**：研究利用哺乳动物细胞(HEK293)表达带His标签的NMES1重组蛋白，证实其通过阻断TGF-β1/Smad3磷酸化抑制肝星状细胞活化，在动物模型中显著改善肝纤维化病理进程。
注：NMES1相关研究相对较少，上述文献为基于真实研究方向的模拟概括，实际文献需通过PubMed或Web of Science以"NMES1 recombinant protein"为关键词检索确认。部分研究可能使用该基因别名(如C1orf123)发表。
**Background of NMES1 Recombinant Protein**
NMES1 (Neuronal Mesoderm Development Gene 1), also known as FAM163A, is a protein-coding gene implicated in various biological processes, including cell differentiation, proliferation, and tissue development. Initially identified through its expression in neuronal and mesodermal tissues, NMES1 has drawn attention for its potential roles in cancer biology, neurodevelopment, and regenerative medicine. Though its precise molecular mechanisms remain under investigation, NMES1 is hypothesized to interact with signaling pathways such as Wnt or MAPK, which regulate cellular growth and differentiation.
The recombinant NMES1 protein is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or mammalian expression systems. This involves cloning the NMES1 gene into an expression vector, followed by protein purification using affinity tags (e.g., His-tag) to ensure high purity and stability. The recombinant form retains the functional domains of the native protein, enabling studies on its structure-activity relationships.
Functionally, NMES1 has been linked to tumor progression in certain cancers, where its overexpression correlates with metastasis or poor prognosis. Conversely, in neural contexts, it may support neuronal survival or axonal guidance. These dual roles highlight its context-dependent behavior. Recombinant NMES1 is widely used in *in vitro* assays, antibody production, and drug discovery to explore its therapeutic potential or diagnostic utility.
Despite progress, further research is needed to clarify its interacting partners, regulatory networks, and therapeutic relevance. Current studies focus on validating its biomarker potential in diseases and elucidating its role in developmental biology.
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