| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENTa1 |
| Uniprot No | O75689 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-374aa |
| 氨基酸序列 | MAKERRRAVLELLQRPGNARCADCGAPDPDWASYTLGVFICLSCSGIHRNIPQVSKVKSVRLDAWEEAQVEFMASHGNDAARARFESKVPSFYYRPTPSDCQLLREQWIRAKYERQEFIYPEKQEPYSAGYREGFLWKRGRDNGQFLSRKFVLTEREGALKYFNRNDAKEPKAVMKIEHLNATFQPAKIGHPHGLQVTYLKDNSTRNIFIYHEDGKEIVDWFNALRAARFHYLQVAFPGAGDADLVPKLSRNYLKEGYMEKTGPKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSKESGYTVLHGFPPSTQGHHWPHGITIVTPDRKFLFACETESDQREWVAAFQKAVDRPMLPQEYAVEAHFKHKP |
| 预测分子量 | 43,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CENTa1重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库获取真实文献)：
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1. **文献名称**：*"High-yield Expression and Functional Analysis of Recombinant CENTa1 in Eukaryotic Systems"*
**作者**：Chen L, Wang T, et al.
**摘要**：研究利用哺乳动物表达系统成功制备了CENTa1重组蛋白，验证了其与染色体着丝粒区域的结合能力，并证实其在细胞分裂过程中调控染色体分离的功能。
2. **文献名称**：*"Structural Characterization of CENTa1 Recombinant Protein via Cryo-EM"*
**作者**：Martinez R, Singh A, et al.
**摘要**：通过冷冻电镜技术解析了CENTa1重组蛋白的三维结构，揭示了其N端结构域在招募其他着丝粒相关蛋白中的关键作用，为靶向药物设计提供依据。
3. **文献名称**：*"CENTa1 Recombinant Protein as a Novel Tool for Epigenetic Studies"*
**作者**：Kim S, Ito M, et al.
**摘要**：研究开发了带标签的CENTa1重组蛋白，用于染色质免疫共沉淀(ChIP)实验，证明了其在维持染色质表观遗传稳定性中的新机制。
4. **文献名称**：*"Antibody Production Using CENTa1 Recombinant Protein and Its Diagnostic Potential"*
**作者**：Garcia P, et al.
**摘要**：利用纯化的CENTa1重组蛋白制备多克隆抗体，并通过免疫组化验证其在多种肿瘤组织中的特异性表达，提示其作为癌症预后标志物的潜力。
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**注意**：以上为模拟示例，实际文献需通过**PubMed**、**Google Scholar**或**Web of Science**等平台检索关键词(如“CENTa1 recombinant protein”或“CENTa1 functional study”)。
CENTa1 is a recombinant protein derived from the venom of the Centruroides noxius scorpion, a species native to Mexico. It belongs to the scorpion toxin family, specifically targeting voltage-gated sodium (NaV) channels, which play critical roles in neuronal excitability and signal transduction. CENTa1 was engineered through molecular cloning and expression systems (e.g., E. coli or yeast) to improve stability, specificity, and yield compared to native toxins. Its structure includes a conserved cysteine-stabilized alpha-beta (CSαβ) fold, characteristic of scorpion neurotoxins, enabling precise interaction with NaV channels.
This protein selectively binds to NaV1.7. a channel subtype implicated in pain signaling pathways, making it a focal point for analgesic drug development. Researchers leverage CENTa1’s mechanism—modulating channel inactivation—to study NaV1.7’s role in chronic pain disorders and explore therapeutic interventions. Additionally, its recombinant nature allows customization (e.g., tagging for imaging or affinity purification) to enhance experimental versatility.
Beyond pain research, CENTa1 serves as a tool for probing ion channel structure-function relationships and screening NaV-targeted pharmaceuticals. Its high specificity reduces off-target effects, a common challenge in neuropharmacology. Recent studies also investigate its potential in treating epilepsy or cardiac arrhythmias, though clinical applications remain exploratory.
In summary, CENTa1 exemplifies how venom-derived recombinant proteins bridge natural toxin biology and biomedical innovation, offering insights into ion channel physiology and therapeutic targeting.
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