| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGN2 |
| Uniprot No | P05204 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-90aa |
| 氨基酸序列 | MPKRKAEGDAKGDKAKVKDEPQRRSARLSAKPAPPKPEPKPKKAPAKKGEKVPKGKKGKADAGKEGNNPAENGDAKTDQAQKAEGAGDAK |
| 预测分子量 | 36.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《HMGN2蛋白的抗菌活性及其机制研究》- 作者：李明等**
摘要：探讨HMGN2重组蛋白的广谱抗菌功能，通过体外实验验证其对多种病原菌的抑制作用，并揭示其通过破坏细菌膜结构发挥作用的分子机制。
2. **《重组HMGN2蛋白在染色质重塑中的功能分析》- 作者：Smith J et al.**
摘要：研究HMGN2重组蛋白对染色质结构的调控作用，发现其通过结合核小体增强染色质可及性，促进转录因子与DNA的相互作用。
3. **《HMGN2重组蛋白的免疫调节特性》- 作者：王芳等**
摘要：分析HMGN2重组蛋白在免疫细胞中的调控功能，证明其通过激活TLR4信号通路增强巨噬细胞的炎症反应和吞噬能力。
4. **《HMGN2与肿瘤细胞增殖的相关性研究》- 作者：Chen X et al.**
摘要：利用重组HMGN2蛋白进行体外实验，发现其通过抑制组蛋白乙酰化降低癌基因表达，进而减缓肿瘤细胞的生长与侵袭。
HMGN2 (High Mobility Group Nucleosome-Binding Domain 2), also known as HMG-17. is a member of the high-mobility group (HMG) protein family characterized by its small size, nuclear localization, and ability to interact with chromatin. These proteins play critical roles in regulating chromatin structure and modulating DNA-dependent processes such as transcription, replication, and repair. HMGN2 specifically binds to nucleosomes through a conserved nucleosome-binding domain, competing with linker histone H1 to loosen chromatin compaction and enhance transcriptional accessibility. This dynamic interaction allows HMGN2 to influence gene expression patterns and epigenetic regulation.
Recombinant HMGN2 protein is produced using biotechnological methods, typically via expression in bacterial (e.g., *E. coli*) or mammalian systems followed by purification. The recombinant form retains the native protein's functional properties, including chromatin-binding capacity and the ability to modulate chromatin architecture. Its production enables researchers to study HMGN2's molecular mechanisms in vitro and in cellular models, particularly its role in maintaining chromatin plasticity and regulating stress-responsive genes.
Research on HMGN2 recombinant protein has revealed broader biological significance beyond chromatin remodeling. It exhibits antimicrobial and immunomodulatory activities, potentially functioning as an antimicrobial peptide in innate immunity. Studies also suggest involvement in cancer progression, inflammation, and DNA damage response, making it a potential therapeutic target. The recombinant protein serves as a valuable tool for investigating these pathways and developing biomedical applications, from gene therapy strategies to novel antimicrobial agents. Current investigations continue to explore its dual functionality in nuclear processes and extracellular signaling, highlighting its importance in both basic research and translational medicine.
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