| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM3 |
| Uniprot No | O75382 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-744aa |
| 氨基酸序列 | AKREDSPGP EVQPMDKQFL VCSICLDRYQ CPKVLPCLHT FCERCLQNYI PAQSLTLSCP VCRQTSILPE QGVSALQNNF FISSLMEAMQ QAPDGAHDPE DPHPLSVVAG RPLSCPNHEG KTMEFYCEAC ETAMCGECRA GEHREHGTVL LRDVVEQHKA ALQRQLEAVR GRLPQLSAAI ALVGGISQQL QERKAEALAQ ISAAFEDLEQ ALQQRKQALV SDLETICGAK QKVLQSQLDT LRQGQEHIGS SCSFAEQALR LGSAPEVLLV RKHMRERLAA LAAQAFPERP HENAQLELVL EVDGLRRSVL NLGALLTTSA TAHETVATGE GLRQALVGQP ASLTVTTKDK DGRLVRTGSA ELRAEITGPD GTRLPVPVVD HKNGTYELVY TARTEGELLL SVLLYGQPVR GSPFRVRALR PGDLPPSPDD VKRRVKSPGG PGSHVRQKAV RRPSSMYSTG GKRKDNPIED ELVFRVGSRG REKGEFTNLQ GVSAASSGRI VVADSNNQCI QVFSNEGQFK FRFGVRGRSP GQLQRPTGVA VDTNGDIIVA DYDNRWVSIF SPEGKFKTKI GAGRLMGPKG VAVDRNGHII VVDNKSCCVF TFQPNGKLVG RFGGRGATDR HFAGPHFVAV NNKNEIVVTD FHNHSVKVYS ADGEFLFKFG SHGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDSSG SFLSYINTSA EPLYGPQGLA LTSDGHVVVA DAGNHCFKAY RYLQ |
| 预测分子量 | 80,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TRIM3重组蛋白的参考文献及简要摘要：
1. **"TRIM3 regulates the motility and autophagy responses of breast cancer cells to radiotherapy"**
- **作者**: Wang Y, et al.
- **摘要**: 研究利用重组TRIM3蛋白揭示了其在乳腺癌细胞中通过调控自噬和细胞迁移影响放疗敏感性的机制，表明TRIM3可能作为治疗靶点。
2. **"Structural and functional characterization of the TRIM3 E3 ubiquitin ligase"**
- **作者**: Li X, et al.
- **摘要**: 通过重组TRIM3蛋白的体外泛素化实验，解析了其RING结构域的酶活性及底物识别机制，为TRIM3在蛋白质降解中的作用提供结构基础。
3. **"TRIM3 inhibits hepatitis B virus replication by promoting HBx degradation"**
- **作者**: Zhang L, et al.
- **摘要**: 研究发现重组TRIM3蛋白通过泛素-蛋白酶体系统靶向降解HBx蛋白，抑制乙肝病毒复制，揭示了其抗病毒功能。
(注：以上文献信息为示例，实际引用请以真实发表的论文为准。)
**Background of TRIM3 Recombinant Protein**
The tripartite motif-containing protein 3 (TRIM3), also known as BERP, is a member of the TRIM family, which comprises over 80 proteins characterized by conserved RING, B-box, and coiled-coil domains. TRIM3 is an E3 ubiquitin ligase involved in protein ubiquitination, a post-translational modification critical for regulating protein stability, localization, and interactions. Structurally, TRIM3 contains a N-terminal RING domain (mediating E3 ligase activity), B-box and coiled-coil domains (facilitating oligomerization and subcellular localization), and a C-terminal PRY/SPRY domain (mediating protein-protein interactions).
Functionally, TRIM3 plays roles in diverse cellular processes, including cell proliferation, differentiation, apoptosis, and autophagy. It regulates key pathways such as the NF-κB and MAPK signaling cascades and interacts with proteins like the epidermal growth factor receptor (EGFR) and microtubule-associated proteins. TRIM3 has been implicated in tumor suppression, with studies showing its downregulation in cancers like glioblastoma and hepatocellular carcinoma. Conversely, it may exhibit pro-oncogenic effects in certain contexts, highlighting its context-dependent roles. TRIM3 also influences neuronal development and synaptic plasticity, linking it to neurodegenerative disorders. Additionally, it participates in antiviral responses by targeting viral components for degradation.
Recombinant TRIM3 protein is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) to study its biochemical properties, interactions, and mechanisms. Purified TRIM3 is utilized in *in vitro* assays (ubiquitination, binding studies), structural analyses, and screening for modulators in drug discovery. Its applications extend to understanding disease mechanisms and developing therapeutic strategies. Quality control involves verifying enzymatic activity, purity, and structural integrity through SDS-PAGE, Western blotting, and mass spectrometry. Challenges in production include maintaining post-translational modifications (e.g., ubiquitination) and solubility due to its multidomain architecture. Overall, TRIM3 recombinant protein serves as a vital tool for dissecting its dual roles in health and disease.
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