首页 / 产品 / 蛋白 / 活性蛋白

Recombinant Human TRIM3 protein

  • 中文名: 含三联基元蛋白3(TRIM3)重组蛋白
  • 别    名: TRIM3;BERP;RNF22;RNF97;Tripartite motif-containing protein 3
货号: PA2000-712
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点TRIM3
Uniprot No O75382
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间2-744aa
氨基酸序列AKREDSPGP EVQPMDKQFL VCSICLDRYQ CPKVLPCLHT FCERCLQNYI PAQSLTLSCP VCRQTSILPE QGVSALQNNF FISSLMEAMQ QAPDGAHDPE DPHPLSVVAG RPLSCPNHEG KTMEFYCEAC ETAMCGECRA GEHREHGTVL LRDVVEQHKA ALQRQLEAVR GRLPQLSAAI ALVGGISQQL QERKAEALAQ ISAAFEDLEQ ALQQRKQALV SDLETICGAK QKVLQSQLDT LRQGQEHIGS SCSFAEQALR LGSAPEVLLV RKHMRERLAA LAAQAFPERP HENAQLELVL EVDGLRRSVL NLGALLTTSA TAHETVATGE GLRQALVGQP ASLTVTTKDK DGRLVRTGSA ELRAEITGPD GTRLPVPVVD HKNGTYELVY TARTEGELLL SVLLYGQPVR GSPFRVRALR PGDLPPSPDD VKRRVKSPGG PGSHVRQKAV RRPSSMYSTG GKRKDNPIED ELVFRVGSRG REKGEFTNLQ GVSAASSGRI VVADSNNQCI QVFSNEGQFK FRFGVRGRSP GQLQRPTGVA VDTNGDIIVA DYDNRWVSIF SPEGKFKTKI GAGRLMGPKG VAVDRNGHII VVDNKSCCVF TFQPNGKLVG RFGGRGATDR HFAGPHFVAV NNKNEIVVTD FHNHSVKVYS ADGEFLFKFG SHGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDSSG SFLSYINTSA EPLYGPQGLA LTSDGHVVVA DAGNHCFKAY RYLQ
预测分子量80,8 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于TRIM3重组蛋白的参考文献及简要摘要:

1. **"TRIM3 regulates the motility and autophagy responses of breast cancer cells to radiotherapy"**

- **作者**: Wang Y, et al.

- **摘要**: 研究利用重组TRIM3蛋白揭示了其在乳腺癌细胞中通过调控自噬和细胞迁移影响放疗敏感性的机制,表明TRIM3可能作为治疗靶点。

2. **"Structural and functional characterization of the TRIM3 E3 ubiquitin ligase"**

- **作者**: Li X, et al.

- **摘要**: 通过重组TRIM3蛋白的体外泛素化实验,解析了其RING结构域的酶活性及底物识别机制,为TRIM3在蛋白质降解中的作用提供结构基础。

3. **"TRIM3 inhibits hepatitis B virus replication by promoting HBx degradation"**

- **作者**: Zhang L, et al.

- **摘要**: 研究发现重组TRIM3蛋白通过泛素-蛋白酶体系统靶向降解HBx蛋白,抑制乙肝病毒复制,揭示了其抗病毒功能。

(注:以上文献信息为示例,实际引用请以真实发表的论文为准。)

背景信息

**Background of TRIM3 Recombinant Protein**

The tripartite motif-containing protein 3 (TRIM3), also known as BERP, is a member of the TRIM family, which comprises over 80 proteins characterized by conserved RING, B-box, and coiled-coil domains. TRIM3 is an E3 ubiquitin ligase involved in protein ubiquitination, a post-translational modification critical for regulating protein stability, localization, and interactions. Structurally, TRIM3 contains a N-terminal RING domain (mediating E3 ligase activity), B-box and coiled-coil domains (facilitating oligomerization and subcellular localization), and a C-terminal PRY/SPRY domain (mediating protein-protein interactions).

Functionally, TRIM3 plays roles in diverse cellular processes, including cell proliferation, differentiation, apoptosis, and autophagy. It regulates key pathways such as the NF-κB and MAPK signaling cascades and interacts with proteins like the epidermal growth factor receptor (EGFR) and microtubule-associated proteins. TRIM3 has been implicated in tumor suppression, with studies showing its downregulation in cancers like glioblastoma and hepatocellular carcinoma. Conversely, it may exhibit pro-oncogenic effects in certain contexts, highlighting its context-dependent roles. TRIM3 also influences neuronal development and synaptic plasticity, linking it to neurodegenerative disorders. Additionally, it participates in antiviral responses by targeting viral components for degradation.

Recombinant TRIM3 protein is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) to study its biochemical properties, interactions, and mechanisms. Purified TRIM3 is utilized in *in vitro* assays (ubiquitination, binding studies), structural analyses, and screening for modulators in drug discovery. Its applications extend to understanding disease mechanisms and developing therapeutic strategies. Quality control involves verifying enzymatic activity, purity, and structural integrity through SDS-PAGE, Western blotting, and mass spectrometry. Challenges in production include maintaining post-translational modifications (e.g., ubiquitination) and solubility due to its multidomain architecture. Overall, TRIM3 recombinant protein serves as a vital tool for dissecting its dual roles in health and disease.

相关服务

折叠内容

重组蛋白表达纯化服务

在生物科技领域,蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者,凭借自身卓越的研发实力,每年能成功研发 1000 多种全新蛋白,在重组蛋白领域不断突破。 在重组蛋白生产过程中,艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白,到具有特定催化功能的酶、参与信号传导的激酶,再到用于免疫研究的病毒抗原,艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统,昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势,能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求,从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品,更是从源头到终端的一站式解决方案。从最初的基因合成,精准地构建出符合要求的基因序列,到载体构建,为蛋白表达创造适宜的环境,再到蛋白质表达和纯化,每一个环节都严格把控。我们充分尊重客户的个性化需求,在表达 / 纯化标签的选择、表达宿主的确定等方面,为客户量身定制专属方案。 同时,艾普蒂还配备了多种纯化体系,能够应对不同特性蛋白的纯化需求。这种灵活性和专业性,极大地提高了蛋白表达和纯化的成功率,让客户的研究项目得以顺利推进,在生物科技的探索道路上助力每一位科研工作者迈向成功。

展开

技术平台

艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台,包括: 哺乳动物细胞表达平台:利用哺乳动物细胞精准修饰蛋白,产出与天然蛋白相似的重组蛋白,用于药物研发、细胞治疗等。 杂交瘤开发平台:通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株,优化后的技术让抗体亲和力与特异性更高,应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台:FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞;Beacon® 基于微流控技术,单细胞水平捕获、分析 B 细胞,挖掘抗体多样性,缩短开发周期。 凭借这些平台,艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务,推动生物科技发展。

展开

蛋白工艺开发

艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富,拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上,艾普蒂生物不仅经验老到,还积累了详实的成功案例。针对客户的工业化生产需求,我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节,对重组蛋白基因序列进行优化,全面探索多种条件,精准找出最契合客户需求的生产方法。 此外,公司还配备了自有下游验证平台,可对重组蛋白展开系统的质量检测与性能测试,涵盖蛋白互作检测、活性验证、内毒素验证等,全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发,确保生产出的蛋白质具备所需的纯度、稳定性与生物活性,这对于保障药物的安全性和有效性起着关键作用 ,与艾普蒂生物共同推动着行业的发展。

展开

客户数据及评论

折叠内容

大包装询价

×