| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRF |
| Uniprot No | P10586 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1275-1897aa |
| 氨基酸序列 | ILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTP GMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEV NKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQ GPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETCGL IQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPT PILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTV DIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARNLYAHI QKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIM PYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFW RMLWEHNSTIIVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNM PQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHK TKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTL RTQRPAMVQTEDQYQLCYRAALE |
| 预测分子量 | 93 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRF重组蛋白的3篇示例参考文献(注：文献为虚构示例，仅供格式参考)：
1. **文献名称**：*Expression and Functional Characterization of Recombinant PTPRF Catalytic Domain in Mammalian Cells*
**作者**：Zhang, Y. et al.
**摘要**：本研究通过哺乳动物表达系统成功表达并纯化了PTPRF的催化结构域，验证其酪氨酸磷酸酶活性，并证明其对胰岛素受体信号通路的调控作用。
2. **文献名称**：*Crystallographic Analysis of the Extracellular Region of Human PTPRF*
**作者**：Tanaka, K. & Müller, C.E.
**摘要**：利用重组技术表达PTPRF的胞外结构域，解析其晶体结构，揭示其与细胞粘附分子相互作用的特异性结合位点，为靶向药物设计提供结构基础。
3. **文献名称**：*Recombinant PTPRF Protein Attenuates EGFR Signaling in Colorectal Cancer Models*
**作者**：Chen, L. et al.
**摘要**：通过原核系统制备重组PTPRF蛋白，证明其通过去磷酸化EGFR抑制结直肠癌细胞增殖，提示其作为潜在治疗靶点的价值。
如需真实文献，建议通过PubMed或Google Scholar检索关键词“PTPRF recombinant protein”或“LAR phosphatase recombinant expression”获取最新研究。
**Background of PTPRF Recombinant Protein**
PTPRF (Protein Tyrosine Phosphatase Receptor Type F), also known as LAR (Leukocyte Antigen-Related), is a member of the protein tyrosine phosphatase (PTP) family. These enzymes regulate cellular signaling by dephosphorylating tyrosine residues on target proteins, counterbalancing the activity of tyrosine kinases. PTPRF is a receptor-type PTP, characterized by an extracellular domain, a transmembrane region, and two intracellular catalytic domains. The extracellular segment contains immunoglobulin-like and fibronectin type III repeats, suggesting roles in cell-cell or cell-matrix interactions, while the intracellular domains mediate phosphatase activity.
PTPRF plays critical roles in diverse processes, including cell adhesion, growth, differentiation, and insulin signaling. Dysregulation of PTPRF has been linked to metabolic disorders (e.g., diabetes) and cancers, where it may act as a tumor suppressor or promoter depending on context. Its involvement in insulin receptor signaling highlights its potential as a therapeutic target for metabolic diseases.
Recombinant PTPRF protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce functional domains, often the phosphatase-active intracellular region. This recombinant form enables in vitro studies of enzyme kinetics, substrate interactions, and inhibitor screening. Researchers utilize it to dissect PTPRF's structural-functional relationships, regulatory mechanisms, and pathological roles. Challenges in producing active recombinant PTPRF include maintaining proper folding and post-translational modifications, often requiring optimized expression and purification strategies.
Overall, PTPRF recombinant protein serves as a vital tool for understanding tyrosine phosphatase biology and developing targeted therapies for related diseases.
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