| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEC |
| Uniprot No | P42680 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-631aa |
| 氨基酸序列 | MNFNTILEEILIKRSQQKKKTSPLNYKERLFVLTKSMLTYYEGRAEKKYRKGFIDVSKIKCVEIVKNDDGVIPCQNKYPFQVVHDANTLYIFAPSPQSRDLWVKKLKEEIKNNNNIMIKYHPKFWTDGSYQCCRQTEKLAPGCEKYNLFESSIRKALPPAPETKKRRPPPPIPLEEEDNSEEIVVAMYDFQAAEGHDLRLERGQEYLILEKNDVHWWRARDKYGNEGYIPSNYVTGKKSNNLDQYEWYCRNMNRSKAEQLLRSEDKEGGFMVRDSSQPGLYTVSLYTKFGGEGSSGFRHYHIKETTTSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSVKGKNAPTTAGFSYEKWEINPSELTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLTHPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDVLLSMCQDVCEGMEYLERNSFIHRDLAARNCLVSEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEVFTEGRMPFEKYTNYEVVTMVTRGHRLYQPKLASNYVYEVMLRCWQEKPEGRPSFEDLLRTIDELVECEETFGR |
| 预测分子量 | 103 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TEC重组蛋白相关的文献示例(注：文献标题和作者为虚拟示例，实际引用需核实)：
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1. **标题**：*Structural and functional analysis of recombinant TEC kinase domain in cancer signaling*
**作者**：A. Smith et al.
**摘要**：本研究通过大肠杆菌表达系统纯化了人源TEC激酶结构域重组蛋白，解析其晶体结构，揭示了其与底物结合的关键位点，并验证了其在B细胞受体信号通路中的调控作用。
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2. **标题**：*TEC-family kinase recombinant proteins as therapeutic targets in autoimmune diseases*
**作者**：J. Lee & R. Tanaka
**摘要**：文章探讨了重组TEC激酶蛋白(如BTK、ITK)在类风湿性关节炎中的异常活性，并通过体外实验证明靶向抑制重组TEC蛋白可减少炎症因子的释放，提示其作为自身免疫疾病药物开发潜力。
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3. **标题**：*Optimization of recombinant TEC protein production in mammalian expression systems*
**作者**：C. Zhang et al.
**摘要**：研究比较了HEK293和CHO细胞表达TEC重组蛋白的效率和活性差异，提出基于哺乳动物系统的糖基化修饰对维持TEC蛋白功能完整性的必要性，为工业化生产提供参考。
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4. **标题**：*Role of recombinant TEC in platelet activation and thrombosis*
**作者**：M. Gonzalez & K. Patel
**摘要**：通过重组TEC蛋白体外实验，发现其参与调控血小板GPVI信号通路，并证实TEC磷酸化缺陷会导致血栓形成异常，为抗凝血治疗提供新机制。
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**注**：以上为示例性内容，实际文献需根据具体研究方向在PubMed、Web of Science等数据库检索，关键词如“TEC kinase recombinant”或“recombinant TEC protein function”。
**Background of TEC Recombinant Proteins**
The **TEC family** of non-receptor tyrosine kinases, comprising members like **BTK (Bruton’s tyrosine kinase)**, **ITK (IL-2-inducible T-cell kinase)**, and **TXK**, plays critical roles in signaling pathways regulating immune cell development, activation, and function. These kinases are primarily expressed in hematopoietic cells, where they mediate signals from antigen receptors (e.g., B-cell and T-cell receptors) and cytokine receptors. Dysregulation of TEC kinases is linked to immune disorders, cancers, and inflammatory diseases, making them key therapeutic targets. For instance, BTK inhibitors like ibrutinib are widely used to treat B-cell malignancies.
**Recombinant TEC proteins** are engineered versions of these kinases, produced using expression systems such as *E. coli* or mammalian cells. Their production enables detailed biochemical and structural studies, aiding in understanding substrate interactions, activation mechanisms, and inhibitor binding. Recombinant TEC proteins retain functional domains, including the pleckstrin homology (PH) domain for membrane localization, tyrosine kinase catalytic domain, and regulatory regions. These features allow researchers to study kinase activity, post-translational modifications (e.g., phosphorylation), and interactions with signaling adaptors in controlled settings.
Applications of recombinant TEC proteins span **drug discovery** (high-throughput screening for inhibitors), **diagnostic tool development**, and **antibody production**. They also serve as standards in proteomic assays to quantify endogenous kinase levels in diseases. Challenges in their production include maintaining proper folding and post-translational modifications, which are critical for functional studies. Advances in protein engineering, such as codon optimization and fusion tags, have improved yield and stability.
Overall, TEC recombinant proteins are indispensable for unraveling immune signaling pathways and accelerating targeted therapies, highlighting their significance in both basic research and clinical translation.
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