| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TESK1 |
| Uniprot No | Q15569 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-626aa |
| 氨基酸序列 | MAGERPPLRGPGPGPGEVPGEGPPGPGGTGGGPGRGRPSSYRALRSAVSSLARVDDFHCAEKIGAGFFSEVYKVRHRQSGQVMVLKMNKLPSNRGNTLREVQLMNRLRHPNILRFMGVCVHQGQLHALTEYMNGGTLEQLLSSPEPLSWPVRLHLALDIARGLRYLHSKGVFHRDLTSKNCLVRREDRGFTAVVGDFGLAEKIPVYREGARKEPLAVVGSPYWMAPEVLRGELYDEKADVFAFGIVLCELIARVPADPDYLPRTEDFGLDVPAFRTLVGDDCPLPFLLLAIHCCNLEPSTRAPFTEITQHLEWILEQLPEPAPLTRTALTHNQGSVARGGPSATLPRPDPRLSRSRSDLFLPPSPESPPNWGDNLTRVNPFSLREDLRGGKIKLLDTPSKPVLPLVPPSPFPSTQLPLVTTPETLVQPGTPARRCRSLPSSPELPRRMETALPGPGPPAVGPSAEEKMECEGSSPEPEPPGPAPQLPLAVATDNFISTCSSASQPWSPRSGPVLNNNPPAVVVNSPQGWAGEPWNRAQHSLPRAAALERTEPSPPPSAPREPDEGLPCPGCCLGPFSFGFLSMCPRPTPAVARYRNLNCEAGSLLCHRGHHAKPPTPSLQLPGARS |
| 预测分子量 | 106 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TESK1重组蛋白的示例参考文献(文献信息为示例性概括，具体文献需通过学术数据库检索确认)：
1. **文献名称**：*"Cloning and Characterization of TESK1: A Novel Member of the LIM Kinase Family"*
**作者**：T. Ishizaki et al.
**摘要**：本研究首次克隆并鉴定了TESK1基因，证明其编码一种新型丝氨酸/苏氨酸激酶。通过重组蛋白表达，发现TESK1可磷酸化cofilin(一种肌动蛋白解聚因子)，提示其在细胞骨架动态调控中的作用。
2. **文献名称**：*"Recombinant TESK1 Regulates Cell Migration via Cofilin Phosphorylation in TGF-β Signaling"*
**作者**：M. Maekawa et al.
**摘要**：利用大肠杆菌表达系统制备重组TESK1蛋白，研究证实其在TGF-β信号通路中通过磷酸化cofilin调节肌动蛋白重组，从而影响细胞迁移和肿瘤转移过程。
3. **文献名称**：*"Structural Basis of TESK1 Kinase Activation and Substrate Recognition"*
**作者**：J. Zhang et al.
**摘要**：通过重组TESK1蛋白的晶体结构解析，揭示了其激酶结构域的自抑制机制及底物结合位点特征，为设计特异性激酶抑制剂提供了结构依据。
4. **文献名称**：*"TESK1 Recombinant Protein Enhances Integrin-Mediated Cell Adhesion through Cofilin Inactivation"*
**作者**：K. Toshima et al.
**摘要**：研究发现，重组TESK1蛋白通过磷酸化cofilin抑制其活性，增强整合素介导的细胞黏附能力，表明其在细胞外基质相互作用中的关键作用。
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**注**：以上为领域内典型研究方向示例，实际文献需通过PubMed、Google Scholar等平台以关键词“TESK1 recombinant protein”或“TESK1 kinase function”检索获取。
TESK1 (testis-specific kinase 1) is a serine/threonine kinase belonging to the LIM kinase family, initially identified for its predominant expression in testicular germ cells. It shares structural homology with LIMK1/2 but exhibits distinct regulatory mechanisms and substrate preferences. TESK1 plays a critical role in cytoskeletal dynamics by phosphorylating cofilin, an actin-binding protein. Cofilin phosphorylation inhibits its actin-severing activity, stabilizing actin filaments and influencing cell motility, cytokinesis, and morphological changes. This regulatory axis connects TESK1 to processes like cancer metastasis, neuronal development, and immune cell function.
Recombinant TESK1 protein is engineered for in vitro studies to dissect its kinase activity, substrate interactions, and signaling pathways. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant protein retains the catalytic kinase domain and regulatory regions necessary for function. Its production often involves affinity tags (e.g., GST, His-tag) to facilitate purification and detection. Researchers use it to explore TESK1's role in Rho GTPase-mediated signaling, where it integrates cues from pathways like TGF-β or integrin activation to modulate cell adhesion and migration.
Interest in TESK1 has grown due to its dual-specificity (serine/threonine and tyrosine kinase activity) and implications in diseases. Overexpression is linked to tumor invasiveness, while its inhibition reduces metastatic potential in preclinical models. Recombinant TESK1 also aids in drug discovery, enabling high-throughput screening for kinase inhibitors. Despite its testis-specific origin, TESK1 is functionally active in somatic cells, particularly in contexts requiring dynamic cytoskeletal remodeling, making it a versatile tool for cell biology and therapeutic research.
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