| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM1 |
| Uniprot No | P48553 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1259aa |
| 氨基酸序列 | MDASEEPLPPVIYTMENKPIVTCAGDQNLFTSVYPTLSQQLPREPMEWRRSYGRAPKMIHLESNFVQFKEELLPKEGNKALLTFPFLHIYWTECCDTEVYKATVKDDLTKWQNVLKAHSSVDWLIVIVENDAKKKNKTNILPRTSIVDKIRNDFCNKQSDRCVVLSDPLKDSSRTQESWNAFLTKLRTLLLMSFTKNLGKFEDDMRTLREKRTEPGWSFCEYFMVQEELAFVFEMLQQFEDALVQYDELDALFSQYVVNFGAGDGANWLTFFCQPVKSWNGLILRKPIDMEKRESIQRREATLLDLRSYLFSRQCTLLLFLQRPWEVAQRALELLHNCVQELKLLEVSVPPGALDCWVFLSCLEVLQRIEGCCDRAQIDSNIAHTVGLWSYATEKLKSLGYLCGLVSEKGPNSEDLNRTVDLLAGLGAERPETANTAQSPYKKLKEALSSVEAFEKHYLDLSHATIEMYTSIGRIRSAKFVGKDLAEFYMRKKAPQKAEIYLQGALKNYLAEGWALPITHTRKQLAECQKHLGQIENYLQTSSLLASDHHLTEEERKHFCQEILDFASQPSDSPGHKIVLPMHSFAQLRDLHFDPSNAVVHVGGVLCVEITMYSQMPVPVHVEQIVVNVHFSIEKNSYRKTAEWLTKHKTSNGIINFPPETAPFPVSQNSLPALELYEMFERSPSDNSLNTTGIICRNVHMLLRRQESSSSLEMPSGVALEEGAHVLRCSHVTLEPGANQITFRTQAKEPGTYTLRQLCASVGSVWFVLPHIYPIVQYDVYSQEPQLHVEPLADSLLAGIPQRVKFTVTTGHYTIKNGDSLQLSNAEAMLILCQAESRAVVYSNTREQSSEAALRIQSSDKVTSISLPVAPAYHVIEFELEVLSLPSAPALGGESDMLGMAEPHRKHKDKQRTGRCMVTTDHKVSIDCPWSIYSTVIALTFSVPFRTTHSLLSSGTRKYVQVCVQNLSELDFQLSDSYLVDTGDSTDLQLVPLNTQSQQPIYSKQSVFFVWELKWTEEPPPSLHCRFSVGFSPASEEQLSISLKPYTYEFKVENFFTLYNVKAEIFPPSGMEYCRTGSLCSLEVLITRLSDLLEVDKDEALTESDEHFSTKLMYEVVDNSSNWAVCGKSCGVISMPVAARATHRVHMEVMPLFAGYLPLPDVRLFKYLPHHSAHSSQLDADSWIENDSLSVDKHGDDQPDSSSLKSRGSVHSACSSEHKGLPMPRLQALPAGQVFNSSSGTQVLVIPSQDDHVLEVSVT |
| 预测分子量 | 142,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMEM1重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，非真实文献)：
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1. **文献名称**：**"Cloning and Functional Characterization of Human TMEM1 as a Novel Apoptosis-Related Protein"**
**作者**：Saito T, Yamamoto K, Mori H
**摘要**：本研究首次克隆并表达了人源TMEM1基因，发现其编码的跨膜蛋白定位于内质网。通过重组蛋白体外实验，发现TMEM1过表达可抑制细胞凋亡，提示其在细胞死亡调控中的潜在作用。
2. **文献名称**：**"Recombinant TMEM1 Protein Purification and Its Role in Calcium Homeostasis"**
**作者**：Chen L, Wang Y, Zhang M
**摘要**：通过大肠杆菌系统表达并纯化了重组TMEM1蛋白，发现其与细胞内钙离子通道调控相关。实验表明TMEM1可能通过调节内质网钙释放影响细胞应激反应。
3. **文献名称**：**"Structural Analysis of TMEM1 Using Cryo-EM Reveals a Pore-Forming Domain"**
**作者**：Zhang X, Liu R, Li J
**摘要**：利用冷冻电镜解析了重组TMEM1蛋白的高分辨率结构，揭示了其跨膜区存在类似离子通道的孔道结构，为理解其生理功能提供了结构基础。
4. **文献名称**：**"TMEM1 Recombinant Protein Enhances Autophagic Flux in Neurodegenerative Disease Models"**
**作者**：Gupta S, Patel V, Kim D
**摘要**：在神经元细胞中过表达重组TMEM1蛋白，发现其通过促进自噬体形成缓解蛋白聚集毒性，为神经退行性疾病治疗提供了新靶点。
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**说明**：以上文献为假设性示例，实际研究中TMEM1的研究可能较少或涉及不同方向。建议通过学术数据库(如PubMed、Web of Science)以“TMEM1 recombinant protein”“TMEM1 function”等关键词检索最新文献。
TMEM1 (Transmembrane Protein 1) is a conserved multi-pass transmembrane protein encoded by the TMEM1 gene in humans. Initially identified through genomic sequencing, its structural features include 8-10 predicted transmembrane domains, suggesting a role in membrane-associated processes. While its precise molecular function remains under investigation, TMEM1 has been implicated in autophagy regulation, lysosomal activity, and intracellular vesicle trafficking. Studies link it to the formation of vacuole-like structures and membrane remodeling events, potentially influencing cellular homeostasis.
The interest in recombinant TMEM1 protein stems from its emerging association with disease pathways. Elevated TMEM1 expression has been observed in certain cancers, including gliomas and hepatocellular carcinoma, where it may promote tumor progression through autophagy-mediated cell survival. Conversely, TMEM1 deficiency has been connected to neurodevelopmental abnormalities in animal models, hinting at critical roles in neural tissue maintenance.
Recombinant TMEM1 production typically employs eukaryotic expression systems (e.g., HEK293 or insect cells) to preserve post-translational modifications and membrane topology. Purification challenges arise from its hydrophobic nature, often requiring detergent-based solubilization strategies. Current research applications include functional studies of its interactome, structural analysis of transmembrane domains, and screening for modulators in autophagy-related disorders. Its potential as a therapeutic target or biomarker continues to drive recombinant protein development, particularly for neurodegenerative diseases and oncology research where autophagy dysregulation plays a key pathological role.
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