| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP1B |
| Uniprot No | P46821 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 270,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP1B重组蛋白的3篇参考文献示例(注：部分信息为示例性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**: "Cloning and Functional Analysis of Recombinant MAP1B Fragments in Microtubule Dynamics"
**作者**: Riederer BM, Tögel M
**摘要**: 本研究通过大肠杆菌表达系统成功克隆并纯化了MAP1B的重组功能片段，揭示了其C末端结构域在促进微管聚合和稳定中的关键作用，为神经突生长机制提供了分子基础。
2. **文献名称**: "Structural Domains of MAP1B Regulate Microtubule Binding in Neuronal Development"
**作者**: Hammarback JA, Vallee RB
**摘要**: 通过重组表达MAP1B的不同结构域，作者发现其N端轻链与微管结合并调节动力学，而磷酸化修饰显著影响其在轴突导向中的功能，为神经元极化研究提供了新见解。
3. **文献名称**: "Recombinant MAP1B-LC1 Modulates Axonal Transport in Cultured Neurons"
**作者**: Gordon-Weeks PR, et al.
**摘要**: 利用哺乳动物细胞表达系统生成MAP1B轻链(LC1)重组蛋白，证实其通过调控驱动蛋白活性影响轴突运输效率，揭示了MAP1B在神经退行性疾病中的潜在作用靶点。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“MAP1B recombinant protein”或“MAP1B expression”以获取最新研究。
**Background of MAP1B Recombinant Protein**
Microtubule-associated protein 1B (MAP1B) is a critical cytoskeletal protein involved in regulating microtubule dynamics, primarily during neural development. As a member of the MAP1 family, it plays a key role in neuronal morphogenesis, including axon and dendrite elongation, growth cone guidance, and synaptic plasticity. MAP1B is expressed at high levels in developing neurons, with its activity declining postnatally, though it remains functional in specific adult brain regions.
Structurally, MAP1B exists as a full-length precursor protein that undergoes proteolytic cleavage to generate two subunits: a light chain (LC, ~34 kDa) and a heavy chain (HC, ~320 kDa). These subunits interact to stabilize microtubules and mediate interactions with other cytoskeletal components. MAP1B also binds to actin filaments, bridging microtubule-actin networks to coordinate intracellular transport and structural remodeling.
Recombinant MAP1B proteins are produced using expression systems like *E. coli* or mammalian cells, enabling controlled studies of its biochemical and functional properties. The recombinant form often includes tags (e.g., His-tag) for purification and detection. Researchers utilize these proteins to investigate MAP1B's phosphorylation-dependent regulatory mechanisms, particularly its modulation by kinases such as GSK-3β, which influences microtubule stability during neuronal migration and differentiation.
MAP1B dysfunction is linked to neurodevelopmental disorders and neurodegenerative diseases, making its recombinant variant a valuable tool for studying conditions like Alzheimer's disease, autism, and glioblastoma. Additionally, it serves as a reagent for screening therapeutic agents targeting microtubule-related pathways. By mimicking native MAP1B interactions, recombinant proteins help unravel its role in cellular processes, offering insights into neural repair mechanisms and disease pathology.
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