| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | QARS |
| Uniprot No | P47897 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-775aa |
| 氨基酸序列 | AALDSLSLF TSLGLSEQKA RETLKNSALS AQLREAATQA QQTLGSTIDK ATGILLYGLA SRLRDTRRLS FLVSYIASKK IHTEPQLSAA LEYVRSHPLD PIDTVDFERE CGVGVIVTPE QIEEAVEAAI NRHRPQLLVE RYHFNMGLLM GEARAVLKWA DGKMIKNEVD MQVLHLLGPK LEADLEKKFK VAKARLEETD RRTAKDVVEN GETADQTLSL MEQLRGEALK FHKPGENYKT PGYVVTPHTM NLLKQHLEIT GGQVRTRFPP EPNGILHIGH AKAINFNFGY AKANNGICFL RFDDTNPEKE EAKFFTAICD MVAWLGYTPY KVTYASDYFD QLYAWAVELI RRGLAYVCHQ RGEELKGHNT LPSPWRDRPM EESLLLFEAM RKGKFSEGEA TLRMKLVMED GKMDPVAYRV KYTPHHRTGD KWCIYPTYDY THCLCDSIEH ITHSLCTKEF QARRSSYFWL CNALDVYCPV QWEYGRLNLH YAVVSKRKIL QLVATGAVRD WDDPRLFTLT ALRRRGFPPE AINNFCARVG VTVAQTTMEP HLLEACVRDV LNDTAPRAMA VLESLRVIIT NFPAAKSLDI QVPNFPADET KGFHQVPFAP IVFIERTDFK EEPEPGFKRL AWGQPVGLRH TGYVIELQHV VKGPSGCVES LEVTCRRADA GEKPKAFIHW VSQPLMCEVR LYERLFQHKN PEDPTEVPGG FLSDLNLASL HVVDAALVDC SVALAKPFDK FQFERLGYFS VDPDSHQGKL VFNRTVTLKE DPGKV |
| 预测分子量 | 87,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于QARS(谷氨酰-tRNA合成酶)重组蛋白的3篇代表性文献摘要示例：
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1. **文献名称**：*Expression and Characterization of Recombinant Human Glutaminyl-tRNA Synthetase*
**作者**：Smith J, et al.
**摘要**：本研究报道了在大肠杆菌中高效表达并纯化人源QARS重组蛋白的方法。通过优化密码子使用和诱导条件，获得高纯度酶，并验证其催化活性，证明其可用于体外氨基酰化反应研究。
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2. **文献名称**：*Structural Insights into QARS Dysfunction in Neurodegenerative Disorders*
**作者**：Chen L, et al.
**摘要**：利用X射线晶体学解析了重组QARS蛋白的原子结构，揭示了其底物结合域的关键突变位点。实验表明，某些突变导致酶活性降低，可能与遗传性周围神经病变的发生相关。
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3. **文献名称**：*Development of a QARS Inhibitor Screening Platform Using Recombinant Enzyme*
**作者**：Wang Y, et al.
**摘要**：基于重组QARS蛋白建立高通量药物筛选体系，鉴定出两种小分子化合物可特异性抑制酶活性，为治疗QARS过表达相关的肿瘤提供了潜在先导化合物。
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注：以上文献信息为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of QARS Recombinant Protein**
QARS (Glutaminyl-tRNA Synthetase) is a member of the aminoacyl-tRNA synthetase (AaRS) family, enzymes essential for protein synthesis. It catalyzes the attachment of glutamine to its cognate tRNA (tRNA^Gln), ensuring the accurate translation of genetic codes into functional proteins. As a class I AaRS, QARS contains a conserved catalytic domain and plays a critical role in maintaining cellular proteostasis. Dysregulation or mutations in QARS have been linked to neurological disorders, such as progressive microcephaly and intellectual disabilities, highlighting its importance in neural development.
Recombinant QARS protein is produced using biotechnological methods, often through heterologous expression in systems like *E. coli*, yeast, or mammalian cells. This allows large-scale production of the purified enzyme for research and therapeutic applications. The recombinant form retains the enzymatic activity and structural features of native QARS, making it invaluable for studying tRNA charging mechanisms, enzyme kinetics, and interactions with substrates or inhibitors.
In biomedical research, QARS recombinant protein is used to investigate its role in diseases, including cancer and neurodegenerative conditions, where tRNA synthetases may contribute to pathological signaling pathways. Additionally, it serves as a tool for screening potential drugs targeting QARS dysfunction or for developing enzyme replacement therapies. Structural studies using recombinant QARS have also advanced understanding of its substrate-binding sites and conformational dynamics, aiding in rational drug design.
Overall, QARS recombinant protein bridges fundamental biochemistry with translational applications, offering insights into cellular processes and therapeutic strategies for related disorders.
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