| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCN11 |
| Uniprot No | P31025 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-176aa |
| 氨基酸序列 | MKPLLLAVSLGLIAALQAHHLLASDEEIQDVSGTWYLKAMTVDREFPEMN LESVTPMTLTTLEGGNLEAKVTMLISGRCQEVKAVLEKTDEPGKYTADGG KHVAYIIRSHVKDHYIFYCEGELHGKPVRGVKLVGRDPKNNLEALEDFEK AAGARGLSTESILIPRQSETCSPGSD |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于lipocalin家族重组蛋白研究的推测性参考文献示例(请注意，实际文献可能需要针对性检索)：
1. **《重组人LCN11蛋白在精子成熟中的功能研究》- 作者：Zhang et al. (2020)**
摘要：研究通过表达纯化重组人LCN11蛋白，发现其能与精子表面受体结合，可能参与精子活力调控及受精能力提升。
2. **《LCN11重组蛋白的晶体结构解析及配体结合特性》- 作者：Wang & Li (2018)**
摘要：报道了小鼠LCN11重组蛋白的晶体结构，揭示其疏水腔内的脂肪酸结合位点，为靶向药物设计提供结构基础。
3. **《LCN11重组蛋白在卵巢癌诊断中的潜在生物标志物作用》- 作者：Chen et al. (2021)**
摘要：通过ELISA检测发现，卵巢癌患者血清中LCN11重组蛋白水平显著升高，提示其可能作为新型诊断标志物。
4. **《毕赤酵母表达系统优化LCN11重组蛋白生产》- 作者：Kim et al. (2019)**
摘要：开发了高效表达LCN11重组蛋白的毕赤酵母工程菌株，优化后的产量达120 mg/L，适用于规模化制备。
**提示**：以上为模拟内容，实际研究中LCN11相关文献较少，建议通过PubMed/Google Scholar结合关键词(LCN11 + recombinant/production/function)检索最新文献，并确认基因命名准确性。
LCN11 (Lipocalin 11) is a member of the lipocalin protein family, characterized by a conserved eight-stranded β-barrel structure that facilitates interactions with small hydrophobic molecules, such as lipids, hormones, and metabolites. This family plays diverse roles in cellular transport, immune regulation, and signaling. LCN11. though less studied compared to other lipocalins like LCN2 or retinol-binding proteins, is hypothesized to function in lipid metabolism, cell adhesion, or chemoattractant processes due to structural homology and tissue-specific expression patterns.
Genetically encoded in humans on chromosome 9 (9q34.3), LCN11 is expressed in tissues including the prostate, liver, and reproductive organs, suggesting potential roles in endocrine or reproductive biology. Its recombinant form is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified protein for functional studies. Recombinant LCN11 retains the canonical lipocalin fold, enabling ligand-binding assays, structural analyses (e.g., X-ray crystallography), and exploration of its interaction partners.
Current research focuses on elucidating LCN11's physiological ligands and pathways. Preliminary data imply involvement in inflammation modulation and cancer progression, as lipocalins often regulate cell proliferation and apoptosis. Its recombinant variant is also leveraged in diagnostic tool development, given lipocalins' biomarker potential in diseases like prostate cancer. However, mechanistic insights remain limited, necessitating further *in vivo* studies to clarify its biological significance and therapeutic applications. Advances in recombinant technology may accelerate LCN11's utility in drug delivery systems or as a scaffold for engineered proteins.
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