| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYN2 |
| Uniprot No | Q92777 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 348-448aa |
| 氨基酸序列 | AMSDRYKLWVDTCSEMFGGLDICAVKAVHGKDGKDYIFEVMDCSMPLIGE HQVEDRQLITELVISKMNQLLSRTPALSPQRPLTTQQPQSGTLKDPDSSK T |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYN2(Synapsin II)重组蛋白研究的示例参考文献(内容为虚构，仅供格式参考，建议通过PubMed或Google Scholar获取真实文献)：
---
1. **标题**：*Recombinant Expression and Functional Characterization of Synapsin II in Neuronal Vesicle Trafficking*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统表达纯化SYN2重组蛋白，验证其促进突触囊泡聚集的功能，证实其磷酸化修饰对神经递质释放的调控作用。
2. **标题**：*Structural Insights into Synapsin II Dynamics Using Recombinant Protein Crystallography*
**作者**：Chen L, et al.
**摘要**：利用重组SYN2蛋白解析其晶体结构，揭示其N端结构域与ATP结合的分子机制，为神经退行性疾病药物开发提供依据。
3. **标题**：*Synapsin II Knockdown and Recombinant Rescue in a Parkinson’s Disease Model*
**作者**：Garcia R, et al.
**摘要**：在帕金森病细胞模型中，通过外源性添加重组SYN2蛋白恢复突触囊泡循环功能，改善多巴胺能神经元活性。
4. **标题**：*Optimization of Synapsin II Recombinant Production in Mammalian Cell Systems*
**作者**：Kim H, et al.
**摘要**：比较HEK293与CHO细胞表达SYN2重组蛋白的产率与翻译后修饰差异，优化大规模生产方案。
---
**注意**：以上文献为示例，实际研究请查询权威数据库(如PubMed或Web of Science)使用关键词“Synapsin II recombinant protein”或“SYN2 expression”。
**Background of SYN2 Recombinant Protein**
Synapsin II (SYN2), a member of the synapsin protein family, plays a critical role in regulating synaptic vesicle dynamics and neurotransmitter release in neurons. It is primarily expressed in the brain, where it interacts with both synaptic vesicles and the cytoskeleton, modulating synaptic plasticity and maintaining synaptic homeostasis. SYN2 is implicated in fine-tuning presynaptic functions, including vesicle clustering, tethering, and mobilization, thereby influencing synaptic transmission efficiency. Dysregulation of SYN2 has been linked to neuropsychiatric disorders such as schizophrenia and bipolar disorder, highlighting its importance in neural circuit integrity and cognitive functions.
The recombinant SYN2 protein is engineered using advanced molecular biology techniques, often expressed in heterologous systems like *E. coli* or mammalian cell cultures. This approach ensures high purity and specificity, enabling researchers to study SYN2’s structural and functional properties in vitro. Recombinant SYN2 facilitates investigations into its phosphorylation-dependent interactions, its role in vesicle trafficking, and its interplay with other synaptic proteins (e.g., synaptophysin, actin). Additionally, it serves as a vital tool for drug discovery, allowing screening of compounds targeting synaptic dysfunction in neurological diseases.
Recent studies leverage SYN2 recombinant protein to model pathological mechanisms, such as aberrant synaptic connectivity or vesicle pool imbalances, providing insights into therapeutic strategies. Its application extends to antibody production, immunohistochemical assays, and structural biology (e.g., crystallography). By bridging molecular neuroscience and translational research, SYN2 recombinant protein remains pivotal in unraveling synaptic biology and advancing treatments for brain disorders.
×
