| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gABRa1 |
| Uniprot No | P14867 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-251aa |
| 氨基酸序列 | QPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIG |
| 预测分子量 | 29.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GABRA1重组蛋白的3篇参考文献摘要示例(注：文献信息为模拟，仅供参考)：
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1. **文献名称**: *"Expression and purification of functional human GABAA receptor α1 subunit in Escherichia coli"*
**作者**: Smith J, et al.
**摘要**: 本研究报道了利用大肠杆菌系统表达人源GABRA1重组蛋白的优化方法，通过亲和层析和复性技术获得高纯度蛋白，并验证其与配体结合的功能活性，为后续结构研究奠定基础。
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2. **文献名称**: *"Cryo-EM structure of the recombinant α1β2γ2 GABAA receptor in complex with GABA"*
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜技术解析了重组表达的GABAA受体(含GABRA1亚基)与GABA结合的近原子分辨率结构，揭示了配体结合口袋的构象变化及受体激活机制。
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3. **文献名称**: *"Recombinant GABRA1 extracellular domain production in mammalian cells for antibody discovery"*
**作者**: Kim S, et al.
**摘要**: 利用HEK293细胞表达GABRA1胞外域重组蛋白，开发了针对癫痫相关表位的单克隆抗体筛选平台，证明重组蛋白在疾病诊断工具开发中的应用潜力。
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**备注**：实际文献需通过PubMed或Google Scholar检索关键词“GABRA1 recombinant protein”或“GABAA receptor alpha1 subunit expression”获取。如需具体文献，建议补充时间范围或研究主题(如结构、疾病模型等)。
**Background of gABRa1 Recombinant Protein**
The gamma-aminobutyric acid type A receptor subunit alpha-1 (gABRa1) is a critical component of GABAₐ receptors, the primary inhibitory neurotransmitter receptors in the mammalian central nervous system. GABAₐ receptors are ligand-gated ion channels that mediate fast synaptic inhibition, regulating neuronal excitability and contributing to processes such as anxiety regulation, sleep, and seizure control. The alpha-1 subunit, encoded by the *GABRA1* gene, is the most abundant subtype in the brain and is particularly associated with sedative and anxiolytic effects of benzodiazepines, which bind to α-containing GABAₐ receptors.
Recombinant gABRa1 protein is engineered in vitro using heterologous expression systems (e.g., mammalian cells like HEK293 or insect cells) to produce purified, functional subunits for structural and functional studies. This technology allows researchers to study the subunit’s role in receptor assembly, ligand binding, and channel gating without interference from other receptor subtypes. gABRa1 recombinant proteins are often co-expressed with other subunits (e.g., beta and gamma) to reconstitute functional receptors, enabling investigations into receptor pharmacology, allosteric modulation, and disease-linked mutations.
Mutations in *GABRA1* are linked to epilepsy syndromes, including genetic generalized epilepsies and developmental disorders, making recombinant gABRa1 a valuable tool for studying pathogenic mechanisms and screening potential therapeutics. Its applications extend to drug discovery, particularly for benzodiazepine derivatives and novel antiepileptics, as well as structural biology (e.g., cryo-EM studies) to resolve receptor architecture. By providing a controlled platform, recombinant gABRa1 facilitates advances in understanding GABAergic signaling and its dysregulation in neurological and psychiatric diseases.
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