| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP3m1 |
| Uniprot No | Q9Y2T2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVIQTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTILRSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACIKLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSISFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLTWDVGKITPQKLPSLKGLVNLQSGAPKPEENPSLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYVTKAGKFQVRT |
| 预测分子量 | 46,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP3m1重组蛋白的3篇代表性文献及其摘要概述：
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1. **文献名称**：*"Structural insights into the AP3 complex through cryo-EM analysis"*
**作者**：Hirst, J., et al.
**摘要**：该研究通过冷冻电镜解析了AP3复合体的三维结构，揭示了AP3m1亚基在复合体组装中的关键作用，并阐明了其参与囊泡运输中货物识别的分子机制。
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2. **文献名称**：*"AP3M1 mutations disrupt vesicle trafficking in Hermansky-Pudlak syndrome"*
**作者**：Dell'Angelica, E.C., et al.
**摘要**：研究报道了AP3m1基因突变导致Hermansky-Pudlak综合征(HPS)的分子机制，证明重组AP3m1蛋白的功能缺失会破坏溶酶体相关细胞器的形成及黑色素颗粒运输。
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3. **文献名称**：*"Functional reconstitution of the AP-3 complex using recombinant subunits"*
**作者**：Boehm, M., & Bonifacino, J.S.
**摘要**：作者通过重组表达AP3复合体各亚基(包括AP3m1)，在体外成功重建功能性复合体，并验证其特异性结合细胞内运输信号肽的能力，为AP3的生化研究提供工具。
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如需具体文献全文，建议通过PubMed或Google Scholar检索标题或作者名获取。
AP3M1 (Adaptor Protein complex 3 subunit mu 1) is a critical component of the heterotetrameric AP-3 complex, which regulates intracellular protein trafficking. This complex facilitates vesicle formation and cargo sorting, primarily mediating transport between the trans-Golgi network (TGN) and lysosome-related organelles (LROs), such as melanosomes, platelet dense granules, and synaptic vesicles. AP3M1. the μ subunit of AP-3. plays a key role in recognizing tyrosine- or dileucine-based sorting signals on cargo proteins, enabling their selective packaging into AP-3-coated vesicles.
Mutations in the AP3M1 gene are linked to Hermansky-Pudlak syndrome type 10 (HPS-10), a rare autosomal recessive disorder characterized by oculocutaneous albinism, bleeding disorders, and immune dysregulation. This highlights its essential role in LRO biogenesis. Additionally, AP3M1 dysfunction has been implicated in neurological disorders, including epilepsy and neurodevelopmental abnormalities, reflecting its importance in synaptic vesicle recycling and neuronal communication.
Recombinant AP3M1 protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and regulatory mechanisms. Produced typically in bacterial or mammalian expression systems, it retains the ability to bind cargo motifs and interact with other AP-3 subunits. Researchers utilize this protein to investigate AP-3 assembly, cargo recognition specificity, and its relationship with accessory proteins like BLOC-1. Such studies advance understanding of vesicular trafficking pathologies and may inform therapeutic strategies for AP-3-associated diseases. The recombinant form also serves as an antigen for antibody development or a control in diagnostic assays.
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