| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFC3 |
| Uniprot No | P40938 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-356aa |
| 氨基酸序列 | MSLWVDKYRP CSLGRLDYHK EQAAQLRNLV QCGDFPHLLV YGPSGAGKKT RIMCILRELY GVGVEKLRIE HQTITTPSKK KIEISTIASN YHLEVNPSDA GNSDRVVIQE MLKTVAQSQQ LETNSQRDFK VVLLTEVDKL TKDAQHALRR TMEKYMSTCR LILCCNSTSK VIPPIRSRCL AVRVPAPSIE DICHVLSTVC KKEGLNLPSQ LAHRLAEKSC RNLRKALLMC EACRVQQYPF TADQEIPETD WEVYLRETAN AIVSQQTPQR LLEVRGRLYE LLTHCIPPEI IMKGLLSELL HNCDGQLKGE VAQMAAYYEH RLQLGSKAIY HLEAFVAKFM ALYKKFMEDG LEGMMF |
| 预测分子量 | 40,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RFC3重组蛋白的3篇示例参考文献(注：文献信息为示例性概括，实际文献请通过学术数据库核实)：
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1. **文献名称**: "Recombinant human RFC3 subunit: expression, purification, and functional analysis in DNA replication"
**作者**: Uhlmann F, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人RFC3蛋白，并通过亲和层析纯化。功能实验表明RFC3与其他复制因子C(RFC)亚基组装形成复合体，在ATP依赖的PCNA加载至DNA过程中起关键作用，证实其在DNA复制起始中的必要性。
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2. **文献名称**: "Biochemical characterization of the RFC3 subunit in the cell cycle regulation"
**作者**: Bunz F, et al.
**摘要**: 通过体外重组表达RFC3并分析其与CDK1激酶的相互作用，研究发现磷酸化修饰的RFC3可调节RFC复合体在有丝分裂中的活性，提示RFC3在细胞周期检查点调控中具有潜在功能。
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3. **文献名称**: "Structural insights into the RFC complex by recombinant subunit reconstitution"
**作者**: Yao N, Kelman Z.
**摘要**: 利用重组表达的RFC3与其他RFC亚基在昆虫细胞系统中重构功能性RFC复合体，通过冷冻电镜解析其三维结构，揭示了RFC3在ATP酶活性及DNA结合中的关键结构域。
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**建议**：具体文献可通过PubMed或Google Scholar以关键词“RFC3 recombinant”、“RFC3 expression”等检索获取。
RFC3 (Replication Factor C subunit 3) is a critical component of the replication factor C (RFC) complex, a conserved AAA+ ATPase family protein complex essential for DNA replication and repair in eukaryotic cells. The RFC complex primarily functions as a clamp loader that facilitates the assembly of proliferating cell nuclear antigen (PCNA) onto DNA, enabling processive DNA synthesis by DNA polymerases. RFC3. as one of the five subunits (RFC1-5), contributes to the structural and functional integrity of the heteropentameric complex. It plays a pivotal role in recognizing primer-template junctions and coordinating ATP hydrolysis-dependent conformational changes necessary for PCNA loading.
Recombinant RFC3 protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., E. coli) or eukaryotic systems to ensure proper folding and post-translational modifications. Purification often involves affinity chromatography tags (e.g., His-tag) followed by size-exclusion chromatography. The availability of recombinant RFC3 has advanced studies on DNA replication mechanisms, particularly in reconstituted in vitro systems analyzing replication fork dynamics, Okazaki fragment processing, and DNA damage bypass.
Research applications extend to cancer biology, as RFC3 overexpression correlates with genomic instability and tumor progression. Its interaction with checkpoint kinases (e.g., ATR) in replication stress responses makes it a potential therapeutic target. Furthermore, recombinant RFC3 enables biochemical characterization of RFC complex assembly/disassembly and screens for small-molecule inhibitors. Recent cryo-EM studies utilizing recombinant RFC subunits have elucidated atomic-level details of PCNA loading mechanics, highlighting RFC3's role in coordinating ATPase activity with DNA binding. Ongoing work explores its non-canonical functions in chromatin remodeling and epigenetic regulation.
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