| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR157 |
| Uniprot No | Q5UAW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335aa |
| 氨基酸序列 | MQPSPPPTELVPSERAVVLLSCALSALGSGLLVATHALWPDLRSRARRLL LFLSLADLLSAASYFYGVLQNFAGPSWDCVLQGALSTFANTSSFFWTVAI ALYLYLSIVRAARGPRTDRLLWAFHVVSWGVPLVITVAAVALKKIGYDAS DVSVGWCWIDLEAKDHVLWMLLTGKLWEMLAYVLLPLLYLLVRKHINRAH TALSEYRPILSQEHRLLRHSSMADKKLVLIPLIFIGLRVWSTVRFVLTLC GSPAVQTPVLVVLHGIGNTFQGGANCIMFVLCTRAVRTRLFSLCCCCCSS QPPTKSPAGTPKAPAPSKPGESQESQGTPGELPST |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR157重组蛋白的假设性参考文献示例，供参考：
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1. **标题**: *"Cloning and Characterization of Human GPR157: An Orphan G-Protein-Coupled Receptor Enriched in the Brain"*
**作者**: Sugo T, et al.
**摘要**: 本研究成功克隆了人源GPR157基因，并发现其在大脑皮层和海马区高表达。通过重组蛋白表达技术，在哺乳动物细胞中获得了功能性GPR157蛋白，证实其与Gαs蛋白偶联，可能参与cAMP信号通路调控。
2. **标题**: *"Expression and Purification of Recombinant GPR157 in Insect Cells for Structural Studies"*
**作者**: Zhang Y, et al.
**摘要**: 利用杆状病毒-昆虫细胞系统高效表达并纯化GPR157重组蛋白，结合冷冻电镜技术解析其跨膜结构域特征，揭示了其独特的胞外环糖基化修饰可能影响配体结合能力。
3. **标题**: *"GPR157 Regulates Neuronal Differentiation via cAMP/PKA Pathway in vitro"*
**作者**: Smith J, et al.
**摘要**: 通过构建GPR157重组蛋白过表达模型，发现其激活下游cAMP/PKA信号通路，促进神经干细胞向神经元分化，提示其在神经发育中的潜在作用。
4. **标题**: *"Proteomic Analysis of GPR157-Interacting Proteins in Glioblastoma Cells"*
**作者**: Chen L, et al.
**摘要**: 利用重组GPR157蛋白进行免疫共沉淀实验，筛选出胶质瘤细胞中与GPR157相互作用的β-arrestin-1和EGFR等蛋白，为研究其在肿瘤中的功能提供线索。
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**说明**：以上文献为假设示例，实际研究中GPR157的相关文献较少，建议通过PubMed或Web of Science以“GPR157 recombinant”为关键词检索最新进展。
GPR157 is a member of the G protein-coupled receptor (GPCR) superfamily, a large class of transmembrane proteins involved in signal transduction across diverse physiological processes. Despite its classification, GPR157 remains an understudied orphan receptor, meaning its endogenous ligand, downstream signaling pathways, and precise biological functions are not yet fully elucidated. Phylogenetically, it clusters within the adhesion GPCR subfamily, characterized by extracellular domains with adhesion-like motifs, suggesting potential roles in cellular adhesion, development, or tissue homeostasis. Recent studies have detected GPR157 expression in the central nervous system, particularly in the brain’s ventricular zones and choroid plexus, implicating possible involvement in neurodevelopment or cerebrospinal fluid regulation. Its expression patterns during embryogenesis further hint at roles in early tissue organization.
Recombinant GPR157 protein refers to the engineered version of this receptor, typically produced in heterologous systems like mammalian cells (HEK293 or CHO) or insect cells using baculovirus vectors. These systems enable large-scale production of the protein with post-translational modifications critical for structural and functional studies. Recombinant GPR157 is essential for in vitro experiments to deorphanize the receptor, identify binding partners, or map signaling cascades. It also facilitates antibody development, structural biology (e.g., crystallography or cryo-EM), and high-throughput drug screening to uncover therapeutic targets. Challenges in studying GPR157 include low natural abundance and stability issues common to membrane proteins. Advances in protein engineering, solubilization techniques, and lipid-based reconstitution systems are improving research feasibility. As an undercharacterized GPCR, GPR157 represents a frontier for discovering novel signaling mechanisms with potential implications in neurology, oncology, or developmental disorders.
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