| 纯度 | >90%SDS-PAGE. |
| 种属 | Xenopus laevis |
| 靶点 | wnt8 |
| Uniprot No | P28026 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-358aa |
| 氨基酸序列 | AWSVNNFLMTGPKAYLTYSASVAVGAQNGIEECKYQFAWERWNCPESTLQLATHNGLRSATRETSFVHAISSAGVMYTLTRNCSMGDFDNCGCDDSRNGRIGGRGWVWGGCSDNAEFGERISKLFVDGLETGQDARALMNLHNNEAGRLAVKETMKRTCKCHGISGSCSIQTCWLQLAEFRDIGNHLKIKHDQALKLEMDKRKMRSGNSADNRGAIADAFSSVAGSELIFLEDSPDYCLKNISLGLQGTEGRECLQSGKNLSQWERRSCKRLCTDCGLRVEEKKTEIISSCNCKFHWCCTVKCEQCKQVVIKHFCARRERDSNMLNTKRKNRGHRR |
| 预测分子量 | 39.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Wnt8重组蛋白的3-4条参考文献示例(部分信息基于领域内常见研究方向整合，非真实文献)：
1. **文献名称**：*"Recombinant Wnt8 Protein Induces Axis Formation in Zebrafish Embryos"*
**作者**：Hikasa, H., & Sokol, S.Y.
**摘要**：该研究通过表达纯化Wnt8重组蛋白，证明其在斑马鱼胚胎早期发育中促进体轴形成，激活经典Wnt/β-catenin信号通路，并通过胚胎显微注射实验验证其功能。
2. **文献名称**：*"Wnt8-mediated Mesoderm Induction in Xenopus Requires Interaction with Frizzled Receptors"*
**作者**：Leyns, L., et al.
**摘要**：利用重组Wnt8蛋白处理非洲爪蟾胚胎组织，揭示其通过结合Frizzled受体激活下游信号，诱导中胚层分化，并证明其与BMP信号的协同作用。
3. **文献名称**：*"Structural Analysis of Recombinant Wnt8 Reveals Lipid Modification and Receptor Binding Specificity"*
**作者**：Janda, C.Y., et al.
**摘要**：通过X射线晶体学解析重组Wnt8蛋白的结构，发现其棕榈酰化修饰对稳定蛋白构象及结合LRP5/6共受体至关重要，为靶向Wnt通路的药物设计提供依据。
4. **文献名称**：*"Wnt8 Regulates Cancer Stem Cell Properties via Non-canonical Signaling in Human Colorectal Cells"*
**作者**：Nusse, R., & Varmus, H.
**摘要**：在结肠癌细胞系中应用重组Wnt8蛋白，发现其通过非经典Wnt/Ca²⁺通路增强肿瘤干性，提示Wnt8可能成为癌症治疗的潜在靶点。
(注：以上为模拟参考文献，实际文献需通过PubMed/Google Scholar等平台检索确认。)
**Background of Wnt8 Recombinant Protein**
The Wnt family comprises evolutionarily conserved secreted glycoproteins critical for embryogenesis, tissue homeostasis, and cell fate determination. Among them, Wnt8 is a key player in vertebrate development, particularly in axial patterning, neural induction, and mesoderm specification. It is notably involved in the Wnt/β-catenin signaling pathway, where it binds to Frizzled receptors and LRP co-receptors, triggering downstream signals that regulate gene expression. Dysregulation of Wnt8 signaling is linked to developmental abnormalities and diseases, including cancers.
Recombinant Wnt8 protein is engineered *in vitro* using expression systems (e.g., mammalian, insect, or bacterial cells) to produce bioactive forms for research and therapeutic exploration. Its production often faces challenges due to post-translational lipid modifications (e.g., palmitoylation), which are essential for functionality but reduce solubility. To address this, purification strategies may involve detergent-based extraction or fusion tags to enhance stability.
Wnt8 recombinant protein is widely utilized to study early developmental processes, such as gastrulation in model organisms (zebrafish, Xenopus), and to dissect signaling crosstalk in stem cell differentiation or tissue regeneration. It also serves as a tool to modulate Wnt activity in disease models, offering insights into potential therapeutic interventions. Recent studies highlight its role in evolutionary biology, as Wnt8 orthologs exhibit conserved functions across species, reflecting its fundamental role in metazoan development.
In summary, Wnt8 recombinant protein bridges mechanistic studies of developmental biology and translational research, providing a versatile platform to explore Wnt signaling dynamics and its implications in health and disease.
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