| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBX15 |
| Uniprot No | Q96SF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-494aa |
| 氨基酸序列 | MSSMEEIQVELQCADLWKRFHDIGTEMIITKAGRRMFPAMRVKITGLDPHQQYYIAMDIVPVDNKRYRYVYHSSKWMVAGNADSPVPPRVYIHPDSLASGDTWMRQVVSFDKLKLTNNELDDQGHIILHSMHKYQPRVHVIRKDFSSDLSPTKPVPVGDGVKTFNFPETVFTTVTAYQNQQITRLKIDRNPFAKGFRDSGRNRTGLEAIMETYAFWRPPVRTLTFEDFTTMQKQQGGSTGTSPTTSSTGTPSPSASSHLLSPSCSPPTFHLAPNTFNVGCRESQLCNLNLSDYPPCARSNMAALQSYPGLSDSGYNRLQSGTTSATQPSETFMPQRTPSLISGIPTPPSLPGNSKMEAYGGQLGSFPTSQFQYVMQAGNAASSSSSPHMFGGSHMQQSSYNAFSLHNPYNLYGYNFPTSPRLAASPEKLSASQSTLLCSSPSNGAFGERQYLPSGMEHSMHMISPSPNNQQATNTCDGRQYGAVPGSSSQMSVH |
| 预测分子量 | 70.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBX15重组蛋白的3篇代表性文献概览(注：文献信息为模拟示例，实际文献可能需要通过数据库检索确认)：
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1. **文献名称**：*TBX15 regulates skeletal muscle development through modulation of Wnt signaling*
**作者**：Smith J, et al.
**摘要**：研究通过构建TBX15重组蛋白，分析其在体外肌肉细胞分化中的作用，发现其通过调控Wnt信号通路促进成肌细胞增殖和分化，为肌肉发育机制提供新见解。
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2. **文献名称**：*Recombinant TBX15 protein enhances chondrogenic differentiation of mesenchymal stem cells*
**作者**：Lee H, et al.
**摘要**：利用大肠杆菌表达系统纯化TBX15重组蛋白，证明其通过结合特定DNA序列激活软骨形成相关基因(如Sox9)，为软骨再生治疗提供潜在工具。
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3. **文献名称**：*Structural and functional characterization of TBX15 recombinant protein in craniofacial development*
**作者**：Garcia R, et al.
**摘要**：通过昆虫细胞表达系统获得高纯度TBX15重组蛋白，结合X射线晶体学解析其三维结构，揭示其与DNA结合域的关键氨基酸位点，解释部分颅面畸形的分子机制。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“TBX15 recombinant protein”获取最新研究。
**Background of TBX15 Recombinant Protein**
The TBX15 recombinant protein is derived from the T-box transcription factor 15 (TBX15) gene, a member of the evolutionarily conserved T-box gene family. These genes encode transcription regulators characterized by a conserved DNA-binding domain, the T-box, which binds to specific DNA sequences to control embryonic development and tissue differentiation. TBX15 plays critical roles in mesoderm-derived tissues, including skeletal development, adipogenesis, and craniofacial morphogenesis. It is particularly noted for its involvement in regulating progenitor cell differentiation during embryogenesis, influencing processes such as limb patterning and cartilage formation.
Recombinant TBX15 protein is produced using genetic engineering techniques, typically through expression in bacterial (e.g., *E. coli*) or mammalian cell systems. This allows large-scale production of the purified protein for research applications. The recombinant form often includes tags (e.g., His-tag, GST) for easier purification and detection. Researchers utilize TBX15 recombinant protein to study its molecular interactions, DNA-binding properties, and regulatory mechanisms in developmental pathways. It serves as a tool to investigate signaling cascades, such as BMP (bone morphogenetic protein) and Wnt pathways, where TBX15 acts as a mediator.
Dysregulation of TBX15 is linked to congenital disorders, including Cousin syndrome and craniofacial anomalies, highlighting its biomedical relevance. Structural and functional studies using the recombinant protein aim to unravel its role in disease pathogenesis and potential therapeutic targeting. Additionally, TBX15 recombinant protein is employed in *in vitro* assays, cell differentiation studies, and as an antigen for antibody development. Its application extends to regenerative medicine, particularly in skeletal and adipose tissue engineering, where modulating TBX15 activity could enhance tissue repair strategies.
Overall, TBX15 recombinant protein is a vital resource for dissecting developmental biology and exploring translational opportunities in genetic and degenerative diseases.
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