| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GDAP2 |
| Uniprot No | Q9NXN4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496aa |
| 氨基酸序列 | MDPLGAPSQFVDVDTLPSWGDSCQDELNSSDTTAEIFQEDTVRSPFLYNKDVNGKVVLWKGDVALLNCTAIVNTSNESLTDKNPVSESIFMLAGPDLKEDLQKLKGCRTGEAKLTKGFNLAARFIIHTVGPKYKSRYRTAAESSLYSCYRNVLQLAKEQSMSSVGFCVINSAKRGYPLEDATHIALRTVRRFLEIHGETIEKVVFAVSDLEEGTYQKLLPLYFPRSLKEENRSLPYLPADIGNAEGEPVVPERQIRISEKPGAPEDNQEEEDEGLGVDLSFIGSHAFARMEGDIDKQRKLILQGQLSEAALQKQHQRNYNRWLCQARSEDLSDIASLKALYQTGVDNCGRTVMVVVGRNIPVTLIDMDKALLYFIHVMDHIAVKEYVLVYFHTLTSEYNHLDSDFLKKLYDVVDVKYKRNLKAVYFVHPTFRSKVSTWFFTTFSVSGLKDKIHHVDSLHQLFSAISPEQIDFPPFVLEYDARVRSTRSSPSPGMVY |
| 预测分子量 | 72.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GDAP2重组蛋白的3篇参考文献的简要概括(注：内容基于模拟文献，实际研究可能存在差异)：
---
1. **文献名称**：*Molecular Characterization and Functional Analysis of GDAP2 as a Mitochondrial Protein*
**作者**：Smith A, et al.
**摘要**：研究通过重组GDAP2蛋白的表达和纯化，揭示了其在线粒体膜上的定位，并发现其可能参与线粒体动态调控，为神经退行性疾病机制提供了线索。
2. **文献名称**：*GDAP2 Recombinant Protein Interacts with Tubulin and Modulates Axonal Transport*
**作者**：Lee J, et al.
**摘要**：利用大肠杆菌系统表达GDAP2重组蛋白，发现其与微管蛋白结合，可能通过调节轴突运输影响周围神经功能，提示其在神经病变中的潜在作用。
3. **文献名称**：*Expression and Structural Insights of Human GDAP2 in Lipid Metabolism*
**作者**：Wang Y, et al.
**摘要**：通过重组GDAP2的晶体结构解析，发现其结构域与脂质结合相关，实验表明其可能参与细胞内脂质代谢通路，为代谢性疾病研究提供新方向。
---
(注：以上文献为模拟示例，实际引用需核实真实数据库。)
**Background of GDAP2 Recombinant Protein**
GDAP2 (Ganglioside-induced Differentiation-Associated Protein 2) is a mitochondrial protein encoded by the *GDAP2* gene, primarily expressed in neuronal tissues and implicated in cellular differentiation, mitochondrial dynamics, and metabolism. Structurally, GDAP2 contains a conserved glutathione S-transferase (GST)-like domain, suggesting potential roles in redox regulation or detoxification, though its precise enzymatic activity remains unclear. Unlike its paralog GDAP1. which is linked to Charcot-Marie-Tooth disease, GDAP2 has distinct functional and genetic characteristics, though both share associations with mitochondrial networks.
Recent studies highlight GDAP2’s involvement in neuronal development and maintenance. It interacts with mitochondrial fission/fusion machinery, influencing energy metabolism and apoptosis. Dysregulation of GDAP2 has been observed in neurodegenerative conditions and cancers, where altered mitochondrial function contributes to disease progression. For instance, reduced GDAP2 expression correlates with poor prognosis in glioblastoma, potentially due to impaired mitochondrial respiration and increased oxidative stress.
Recombinant GDAP2 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies to dissect its molecular mechanisms. Purified GDAP2 is utilized in binding assays, structural analyses, and screening for interactors or inhibitors. Its recombinant form also aids in exploring therapeutic strategies targeting mitochondrial dysfunction in neurological disorders or cancer. Despite progress, many aspects of GDAP2’s biology, including its substrate specificity and regulatory pathways, remain underexplored, necessitating further research to unlock its diagnostic or therapeutic potential.
×
